Application of metabolomics approach to study of different parts of Mountain Cultivated Ginseng using UHPLC-QTOF/MS
10.16438/j.0513-4870.2016-0325
- VernacularTitle:基于UHPLC-QTOF/MS的植物代谢组学技术鉴别林下山参的不同部位
- Author:
Xiang-wei CHANG
1
;
De-kun LI
2
;
Tong WANG
3
;
Ya-chao WU
1
;
Ying ZHAO
2
;
Da-zheng ZHOU
2
;
Tao ZHANG
4
;
Zheng-liang YE
2
Author Information
1. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
2. Tianjin Tasly Pride Pharmaceutical Co., Ltd., Tianjin 300410, China
3. Tianjin Medical University, Tianjin 300070, China
4. Liaoning Tasly Sentaoshenrong Co., Ltd., Benxi 117206, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Mountain Cultivated Ginseng;
UHPLC-QTOF/MS;
plant metabolomics;
OPLS-DA;
potential marker
- From:
Acta Pharmaceutica Sinica
2016;51(10):1609-
- CountryChina
- Language:Chinese
-
Abstract:
In present study, an ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS)-based plant metabolomics approach was established to investigate the metabolic profiles of the leaves, main root, branch root, and rhizome of Mountain Cultivated Ginseng (MCG). The UHPLC-QTOF/MS data were subjected to principal component analysis (PCA) and orthogonal partial least squared discrimination analysis (OPLS-DA) to find the potential characteristic components of the four parts of MCG in a quick way. The four different parts could be separated into four different groups of phytochemicals according to the PCA scores. The chemical constituents in four parts of MCG were obviously different. The identities of 81 major peaks that were detected in the four parts of MCG and the potential markers were identified by comparison with the reference compounds or were tentatively assigned by matching the retention time,empirical molecular formula and fragment ions with those of the published compounds of the Panax species. This proposed analytical method is fast, accurate, and reliable for differentiating the different parts of MCG. Moreover, this study supplied a new method for the quality evaluation of other Chinese medicinal materials.
