Analysis of lignans and their metabolites derived from Schisandra chinensis and vinegar Schisandra chinensis in rats' plasma, bile, urine and faeces based on UHPLC-QTOF/MS
10.16438/j.0513-4870.2016-0315
- VernacularTitle:基于UHPLC-QTOF/MS技术的生、醋五味子醇提物在大鼠血浆、胆汁、尿液、粪便中药物原形成分及其代谢产物的分析鉴定
- Author:
Lian-lin SU
1
;
Xue CHENG
1
;
De JI
1
;
Li-jun WANG
1
;
Xi-yan DING
1
;
Tu-lin LU
1
;
Chun-qin MAO
1
;
Ji ZHANG
1
Author Information
1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Schisandra chinensis;
processing with rice vinegar;
UHPLC-QTOF/MS;
lignans;
metabolite
- From:
Acta Pharmaceutica Sinica
2016;51(10):1600-
- CountryChina
- Language:Chinese
-
Abstract:
UHPLC-QTOF/MS technique was used to study the differences of lignans and their metabolites derived from Schisandra chinensis and vinegar Schisandra chinensis in rat plasma, bile, urine and faeces by the data processing techniques such as the dynamic background subtract (DBS), mass defect filtering (MDF) and enhance peak list (EPL) in analysis. In order to enhance accuracy for Schisandra chinensis hepatoprotective effect, we established rat acute alcoholic liver injury model in this experiment, and studied the prototype components and metabolisms of Schisandra lignans in vivo under pathological condition. The main ingredients of alcohol extract are lignans, including deoxyschizandrin, schisandrin B, schizandrin C, schizandrol, schizandrol B, schisantherin, schisantherin B, schisanhenol, gomisin G, gomisin J. The metabolic transformation of lignans in rats was mainly induced by methylation, hydroxyl, oxidation, and so on. Finally, we identified 6 kinds of prototype components and their 20 potential metabolites in Schisandra chinensis group and vinegar Schisandra chinensis group.
