Bioavailability in vivo and in vitro transepithelial transport across Caco-2 cell monolayer of pueraria flavonoids bio-adhesive and floating pellets
10.16438/j.0513-4870.2016-0146
- VernacularTitle:葛根总黄酮生物黏附漂浮微丸体外Caco-2细胞转运和体内生物利用度评价
- Author:
Ying LI
1
;
Yi-qun SONG
1
;
Ya-li HE
1
;
Chun-yan ZHU
1
Author Information
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
pueraria flavonoids;
bio-adhesive and floating pellet;
Caco-2 cell monolayer;
transepithelial transport;
bioavailability
- From:
Acta Pharmaceutica Sinica
2016;51(7):1144-
- CountryChina
- Language:Chinese
-
Abstract:
To study the bioavailability of pueraria flavonoids bio-adhesive and floating pellets, the absorption of puerarin was studied using Caco-2 cell monolayer by liquid chromatography (HPLC) method, comparing the Papp of pueraria flavonoids bio-adhesive and floating pellets with different bio-adhesive materials. Drugs were administered at a dose of 100 mg·kg-1 via ig. The plasma concentration of puerarin was determined by HPLC, the pharmacokinetics were calculated with the WinNonlin 6.0 software. The results showed that the Papp of bio-adhesive and floating pellets with hydroxypropyl methylcellulose (HPMC)-cabomer was largest, which had a significant difference (P<0.05). The AUC0-t of pueraria flavonoids bio-adhesive and floating pellets was 1.79 times of pueraria flavonoids, the Cmax of pueraria flavonoids bio-adhesive and floating pellets and pueraria flavonoids had a significant difference (P<0.05). What's more the MRT had prolonged. In conclusion, pueraria flavonoids bio-adhesive and floating pellets with HPMC-cabomer could significantly facilitate the transport of puerarin on Caco-2 cellular monolayers. The bioavailability of pueraria flavonoids bio-adhesive and floating pellets with HPMC-cabomer was increased more than pueraria flavonoids with a sustained release effect.
