Effect of Bupleurum polysaccharides on splenocytes from lupus like mouse re-challenged with Campylobacter jejuni-S131 in vitro
10.16438/j.0513-4870.2016-0052
- VernacularTitle:柴胡多糖对空肠弯曲菌离体再刺激狼疮样小鼠脾细胞作用研究
- Author:
Hai-xia CHEN
1
;
Wen LI
1
;
Chun-jiao ZHOU
1
;
Hong LI
1
;
Yun-yi ZHANG
1
;
Dao-feng CHEN
2
Author Information
1. Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China
2. Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Bupleurum polysaccharide;
lupus;
Campylobacter jejuni-S131;
total immunoglobulin;
antidsDNA antibody;
interleukin-10;
interferon-γ
- From:
Acta Pharmaceutica Sinica
2016;51(7):1098-
- CountryChina
- Language:Chinese
-
Abstract:
Mice were immunized with Campylobacter jejuni-S131(CJ-S131) to establish the lupus-like model. Splenocytes from lupus like mice were challenged with CJ-S131 to induce inflammatory response in vitro. Bupleurum smithii var. parvifolium polysaccharides (BPs) was added in the inflammatory model to observe its underlying mechanisms of action on lupus. BALB/c mice were randomly divided into three groups including normal control group, adjuvant control group and lupus-like model. Mice were immunized on Day 0 and 14 with CJ-S131 to establish lupus-like syndrome, and sacrificed on Day 19. Splenocytes from each group were collected and divided into blank control group, BPs added group (BPs 5, 10, 20, 40 μg·mL-1), CJ-S131 stimulated group, and CJ-S131 plus BPs group. The levels of total IgG, anti-dsDNA antibody, interferon-γ, interleukin-10(IL-10) and IL-17 were quantified by ELISA. The proliferation of splenocytes was determined in the MTT assay. BPs significantly suppressed the high levels of total IgG, anti-dsDNA antibody, IFN-γ and IL-10 stimulated by CJ-S131 and had no significant effects on increased IL-17 secretion and splenocytes proliferation. The results suggest that re-stimulation of splenocytes with CJ-S131 could establish an inflammatory model in vitro. The effect of BPs on lupus might is related to its inhibition of the production of autoantibodies and associated cytokines.
