Application of the affinity ultrafiltration coupled with LC-MS technology in screening active components of traditional Chinese medicines
10.16438/j.0513-4870.2015-1021
- VernacularTitle:亲和超滤结合液质技术在中药有效成分发现中的应用
- Author:
Si-qi WU
1
;
Hua YANG
1
;
Ping LI
1
Author Information
1. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China
- Publication Type:REVIEWS
- Keywords:
affinity ultrafiltration;
LC-MS;
traditional Chinese medicine;
active components screening
- From:
Acta Pharmaceutica Sinica
2016;51(7):1060-
- CountryChina
- Language:Chinese
-
Abstract:
Affinity ultrafiltration coupled with liquid chromatography-mass spectrometry (LC-MS) technology is a rapid, simple and effective method for discovering active components, which is especially applicable to the complex extracts of traditional Chinese medicines (TCMs). Affinity ultrafiltration can facilitate the rapid separation of small-molecule ligands from the target macromolecules, and LC-MS contributes to the structural identification of potential active compounds. Combination of the two techniques is vital to reveal the effective materials of TCMs and helpful for the TCM-based drug discovery. In this review, according to the studies in recent years, we make a brief introduction about the application of the ultrafiltration coupled with LC-MS technology in screening active components of TCMs, including the principle, characteristics and the research advancement.
