Determination of 14 components in Bazibushen capsule by UPLC-ESI-MS/MS
10.16438/j.0513-4870.2015-0799
- VernacularTitle:UPLC-ESI-MS/MS法测定八子补肾胶囊中14种成分的含量
- Author:
Geng-shen SONG
1
;
Li ZHOU
2
;
Yan-mei XU
3
;
Xi-ru ZHANG
3
;
Lan-tong ZHANG
1
Author Information
1. Hebei Medical University, Shijiazhuang 050017, China
2. CSPC Zhongqi Pharmaceutical Technology(Shijiazhuang) Co. Ltd., Shijiazhuang 050041, China
3. Hebei Institute for Drug Control, Shijiazhuang 050011, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Bazibushen capsule;
quantification;
UPLC-ESI-MS/MS
- From:
Acta Pharmaceutica Sinica
2016;51(5):792-
- CountryChina
- Language:Chinese
-
Abstract:
The study developed a method for the determination of 14 components in Bazibushen capsule by UPLC-ESI-MS/MS. Waters ACQUITY BEH C18 column (50 mm×2.1 mm, 1.7 μm) was used and the column temperature was 40℃. A linear gradient elution of eluents A (acetonitrile) and B (0.1% acetic acid) was used for the separation. The source temperature was set at 150℃. The capillary voltage was set at 2.0 kV. The source offset voltage was kept at 50 V. The desolvation temperature was set at 500℃. The desolvation flow was 800 L·h-1. The cone flow was 150 L·h-1. The nebuliser pressure was 7.0 Bar. Multiple reaction monitoring mode (MRM) is adopted. All of the 14 components showed good linearity (r2 > 0.999 1) in the test ranges. The LOQs for the compounds ranged from 0.11-4.52 ng·mL-1, respectively. The RSDs were 0.8%-2.1%. The overall recoveries were between 97.89% and 101.9% for all compounds. The method is simple, rapid, accurate and highly reproducible, and may be used in the determination of 14 components in Bazibushen capsule.
