Determination of eight active components in Radix Hedysari by HPLC and its cluster analysis
10.16438/j.0513-4870.2015-0737
- VernacularTitle:HPLC同时测定红芪中8个活性成分的含量及聚类分析
- Author:
Xiao-ya LIU
1
;
Ji-ping LI
1
;
Xin-yue CHEN
1
;
Ya-li CHEN
1
;
Yan HONG
1
;
Shi-lan FENG
1
Author Information
1. College of Pharmacy, Lanzhou University, Lanzhou 730000, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
HPLC;
Radix Hedysari;
adenosine;
γ-aminobutyric acid;
flavonoids;
content determination;
clustering analysis
- From:
Acta Pharmaceutica Sinica
2016;51(5):786-
- CountryChina
- Language:Chinese
-
Abstract:
An HPLC method was established for the determination of adenosine, γ-aminobutyric acid (GABA) and six flavonoids (calycosin-7-glucoside, ononin, calycosin, isoliquiritigenin, formononetin and medicarpin) in Radix Hedysari. The samples were extracted with methanol by refluxing for 4 h. The HPLCDAD was performed on a Diamonsil C18 column (250 mm×4.6 mm, 5 μm) with acetonitrile-water as the mobile phase. The column temperature was at 40℃ and the flow rate was 1.0 mL·min-1, while the temperature of drift tube was 110.5℃ and the nebulizing gas flow was 3.1 L·min-1 for the ELSD system. The results showed all the eight components had good linear relationships (r2=0.992 8-1.000 0) in the range of the test concentration. The RSD of precision, stability and repeatability were less than 2%. The average recovery rates were 96.78%-103.45%, and RSD were 0.29%-1.61%. The index component contents of Radix Hedysari form 24 different origins were determined and used as variable factors in clustering analysis. The results were classified into 2 groups basically in accordance with the regional cluster. And the consequence was in consistent with the results of principal component analysis. This HPLC method is simple, shows good sensitive and accurate, and provides the experimental basis for multi-index control of Radix Hedysari. Clustering analysis for Radix Hedysari quality control has a certain reliability and objectivity.
