Simultaneous determination of 4 prenylflavonoids of Chuankezhi injection in rat plasma by LC-MS/MS
10.16438/j.0513-4870.2015-0721
- VernacularTitle:LC-MS/MS法同时测定大鼠血浆中4种淫羊藿黄酮苷
- Author:
Shun-jun XU
1
;
Ya-ling ZHU
2
;
Jie-jing YU
3
;
Yi FENG
3
;
Shuai SUN
1
;
Yi-juan XU
1
;
Liu YANG
3
Author Information
1. Guangzhou ImVin Pharmaceutical Co. Ltd., Guangzhou 510663, China
2. Guangdong Second Traditional Chinese Medical Hospital, Guangzhou 510095, China
3. Guangdong Provincial Hospital of TCM, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510120, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
LC-MS/MS;
Chuankezhi injection;
8-isoprenyl flavonoid glycoside;
plasma concentration;
quantitative analysis
- From:
Acta Pharmaceutica Sinica
2016;51(4):637-
- CountryChina
- Language:Chinese
-
Abstract:
A quantitative method for epimedin A, B, C and icariin in rat plasma was established using LC-MS/MS after intermuscular administration of Chuankezhi injection to rat. Chromatographic separation was performed on an Agilent Eclipse XDB-C18 column (150 mm×2.1 mm, 5.0 μm) at 40℃. Mobile phase consisted of acetonitrile -0.1% formic acid in water (35:65), and the flow rate was 0.22 mL·min-1. The LC effluent was detected and analyzed using an ESI-triple quadrupole tandem mass spectrometer under the multiple reaction monitoring (MRM) in the negative ion mode. The plasma samples were treated with solid phase extraction prior to LC-MS/MS analysis. As a result, all of the four analytes displayed a good linearity over the concentration of 1-1000 ng·mL-1. The RSDs of intra-day and inter-day assays were less than 5.99% and 10.16%, respectively. The relative recovery of each analyte was between 88.1%-101.1% with RSD<7.9% and the absolute recovery was between 72.0%-86.6% (RSD<6.3%). In conclusion, the established method shows good specificity, sensitivity and efficiency for quantifying the four flavonoid glycosides contained in rat plasma.
