Effects of chronic alcohol exposure on hippocampus and cerebral cortex neurons in mice
10.16438/j.0513-4870.2015-0745
- VernacularTitle:长期酒精暴露对小鼠海马及大脑皮质神经细胞的影响
- Author:
Zhan-jun CUI
1
;
Kai-bing ZHAO
2
;
Jin-bo DENG
1
Author Information
1. Institute of Neurobiology of Medical College of Henan University, Kaifeng 475001, China
2. Medical College of Kaifeng University, Kaifeng 475001, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
alcohol exposure;
cerebral cortex;
laser confocal microscope;
immunofluorescence;
DiI scattering
- From:
Acta Pharmaceutica Sinica
2016;51(4):573-
- CountryChina
- Language:Chinese
-
Abstract:
This study was performed to investigate the changes of the number, morphology and ultrastructure of the central nervous system of mice during the long-term alcohol exposure. Mice at 60 days in age were used to establish the long-term alcohol exposure model. The structure of the central nervous system, such as nuclear antigen, dendritic spines and synapses, were labeled by the methods of immunocytochemistry and DiI (1, 1'-dioctadecyl-3, 3, 3', 3'-tetramethy lindocarbocyanine perchlorate) scattering. The results showed that prolonged alcohol exposure could promote apoptosis of nerve cells in the central nervous system, and inhibit the proliferation of neural stem cells, which reduced the number of nerve cells in the central nervous system. Long-term ethanol exposure can also lead to a decrease in the density of dendritic spines of neuron, a smaller number of synapses (connections between nerve cells), and some changes in synaptic ultrastructure. The density of nerve cells and their dendritic spines, as well as the changes of synaptic ultrastructure, suggest that the function of nerve cells may be low.
