Effect of lupeol on migration and invasion of human breast cancer MDA-MB-231 cells and its mechanism
10.16438/j.0513-4870.2015-0948
- VernacularTitle:羽扇豆醇对人乳腺癌MDA-MB-231细胞侵袭转移作用及机制研究
- Author:
Ming WANG
1
;
Hong-xia CUI
2
;
Chao SUN
2
;
Gang LI
2
;
Hong-lan WANG
2
;
Chun-hui XIA
2
;
Yu-chun WANG
2
;
Ji-cheng LIU
2
Author Information
1. Heilongjiang University of Chinese Medicine, Harbin 150040, China
2. Qiqihar Medical College, Qiqihar 161006, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
lupeol;
breast cancer cell;
migration;
invasion
- From:
Acta Pharmaceutica Sinica
2016;51(4):558-
- CountryChina
- Language:Chinese
-
Abstract:
In this study, we examined the inhibitory effects of lupeol, an extract of Euphorbia fischerana Steud, on human breast cancer MDA-MB-231 cells migration and invasion. Lupeol was found to inhibit the invasion of MDA-MB-231 in the cell adhesion assay, transwell test and wound healing assay. The expression of cyclooxygenase-2(COX-2), matrix metalloproteinase-2(MMP-2), -9(MMP-9) and nuclear transcription factor-kappa B (NF-κB) p65 in breast cancer following treatment with different concentrations of lupeol was analyzed with Western blot. Lupeol inhibited the migration and invasion of MDA-MB-231 cells in a dosedependent manner in vitro (P<0.05). The expression of COX-2, MMP-2, MMP-9 and NF-κB p65 levels was significantly down-regulated. These observations suggest that lupeol can inhibit the abilities of invasion of MDA-MB-231 cells by inhibiting the protein expression of COX-2, MMP-2 and MMP-9. Its mechanism may be related to inhibition of the nuclear NF-κB signal pathway.
