An HPLC-MS/MS method for the determination of trans-ferulic acid in human plasma
10.16438/j.0513-4870.2015-0559
- VernacularTitle:HPLC-MS/MS法测定人血浆中反式阿魏酸
- Author:
Xiao-bing LI
1
;
Fu-guo SHI
2
;
Xiao-jing HE
1
;
Ling-yan JIAN
1
;
Li DING
2
Author Information
1. Department of Pharmacy, Shengjing Hospital of China Medical University, Shenyang 110004, China
2. Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
trans-ferulic acid;
photochemical stability;
HPLC-MS/MS;
human plasma;
pharmacokinetics
- From:
Acta Pharmaceutica Sinica
2016;51(3):450-
- CountryChina
- Language:Chinese
-
Abstract:
In this study, we developed a sensitive and rapid HPLC-MS/MS method for the determination of trans-ferulic acid (trans-FA) in plasma samples, and investigated the pharmacokinetics characteristics in healthy volunteers. The plasma samples were extracted with acetic ether, and then separated on a Hedera ODS-2 column with a mobile phase of methanol and 5 mmol·L-1 ammonium acetate buffer solution containing 0.05% acetic acid (34:66) at a flow rate of 0.4 mL·min-1. Electrospray ionization source was applied and operated in the positive ion mode using MRM. The method exhibited a good linearity over the concentration range of 0.1-5 ng·mL-1 (r ≥ 0.9992). The values on both the occasions (intra-and inter-day) were all within 9.2%, and the accuracy was 95.4%-111.4%. No matrix effect and carry-over effect were observed. Trans-FA was stable in human plasma under different storage conditions. The developed HPLC-MS/MS method is rapid, sensitive, accurate, and reproducible, and suitable for the pharmacokinetic study of trans-FA in healthy Chinese volunteers.
