Comparison study of determination methods of free amino acid in Shuxuetong injection by HPTLC, pre-column derivation-UPLC, and ion chromatography
10.16438/j.0513-4870.2015-0623
- VernacularTitle:高效薄层色谱、柱前衍生-超高效液相色谱和离子色谱分析疏血通注射液中游离氨基酸的比较
- Author:
Fei-fei WANG
1
;
Yu-mei ZHANG
1
;
Shuang-cheng MA
1
;
Zhong DAI
1
;
Bin LIU
2
Author Information
1. National Institutes for Food and Drug Control, Beijing 100050, China
2. Beijing University of Chinese Medicine, Beijing 100029, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Shuxuetong injection;
free amino acid;
high performance thin layer chromatography;
pre-column derivatization-ultra performance liquid chromatography;
ion chromatography
- From:
Acta Pharmaceutica Sinica
2016;51(3):439-
- CountryChina
- Language:Chinese
-
Abstract:
Free amino acids play a great role in traditional Chinese medicine in injections of animal products, as they may take part in peptide synthesis and exhibit a bioactivity in vivo. However, most of the national standards for drugs and peer-reviewed papers only focus on the total amount of amino acids after peptide hydrolysis. We compare the advantage and disadvantage among high performance thin layer chromatography (HPTLC), pre-column derivatization ultra performance liquid chromatography (UPLC) and ion chromatography. As a result, the HPTLC and pre-column derivatization UPLC are suitable for quality analysis, while there is high matrix influence for ion chromatography analysis. The verified pre-column derivatization UPLC method is utilized in quantitative analysis. 24 kinds of amino acid were detected by this method, and 8 of them were reported for the first time from the injection. The system has high repeatability and accuracy with LOD on the level of pmol·mL-1.
