Effects and mechanisms of Xiao-Ai-Ping injection on angiogenesis
10.16438/j.0513-4870.2015-0988
- VernacularTitle:消癌平注射液抗血管生成作用及其机制研究
- Author:
Mei-jian WANG
1
;
Dan-yu DU
2
;
Wei FAN
2
;
Cang ZHANG
3
;
Yang LIU
3
;
Jia-hong FAN
2
;
Sheng-tao YUAN
2
;
Sen-sen LIN
2
Author Information
1. Jiangxi Cancer Hospital, Nanchang 330029, China
2. Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China
3. Nanjing Sanhome Pharmaceutical Co., Ltd., Nanjing 210038, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Xiao-Ai-Ping injection;
cancer;
angiogenesis;
vascular endothelial growth factor;
protein kinase B
- From:
Acta Pharmaceutica Sinica
2016;51(2):309-
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to investigate the effect of Xiao-Ai-Ping injection on cancer angiogenesis. CCK8 assay and BrdU incorporation immunofluorescence assay were used to detect the effect of Xiao-Ai-Ping injection on HUVECs proliferation; wound healing assay and transwell assay were employed to test the effect of Xiao-Ai-Ping injection on HUVECs migration. The anti-angiogenic effect of Xiao-Ai-Ping injection was examined by tube formation assay, rat aortic ring assay and chicken chorioallantoic membrane (CAM) assay. ELISA assay was used to measure the secretion of vascular endothelial growth factor (VEGF); and the activation of vascular endothelial growth factor receptor 2 (VEGFR2) protein and its downstream signaling pathways were examined by Western blot. Our data demonstrated that Xiao-Ai-Ping injection inhibited HU-VECs proliferation in a time-and dose-dependent manner, and the IC50 (mg·mL-1) values for 24, 48 and 72 h were 48.7±7.14, 29.1±2.25 and 22.0±4.53, individually. Xiao-Ai-Ping injection inhibited HUVECs DNA synthesis and migration. Xiao-Ai-Ping injection suppressed HUVECs tube formation, and reduced microvessel sprouting from rat aortic rings and vessel growth in CAMs. Furthermore, Xiao-Ai-Ping injection attenuated the secretion of VEGF, and inhibited the expression of p-VEGFR2 and phosphorylation of protein kinase B (p-AKT). We conclude that Xiao-Ai-Ping injection inhibits angiogenesis by down-regulation of VEGF signaling and AKT pathway.
