Evaluation of genetic diversity and population structure of Bletilla striata based on SRAP markers
10.16438/j.0513-4870.2015-0414
- VernacularTitle:基于SRAP标记的白芨遗传多样性和遗传结构评价
- Author:
Yu-long SUN
1
;
Bei-wei HOU
1
;
Li-xia GENG
1
;
Zhi-tao NIU
1
;
Wen-jin YAN
1
;
Qing-yun XUE
1
;
Xiao-yu DING
1
Author Information
1. College of Life Sciences, Nanjing Normal University, Nanjing 210023, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Bletilla striata;
sequence related amplified polymophism;
genetic diversity;
population structure;
conservation
- From:
Acta Pharmaceutica Sinica
2016;51(1):147-
- CountryChina
- Language:Chinese
-
Abstract:
Bletilla striata has been used as traditional Chinese medicine for several centuries. In recent years, the quality and quantity of wild B. striata plants have declined sharply due to habitat deterioration and human over-exploitation. Therefore, it is of great urgency to evaluate and protect B. striata wild plant resource. In this study, sequence-related amplified polymorphism (SRAP) markers were applied to assess the level and pattern of genetic diversity in twelve populations of B. striata. The results showed a high level of genetic diversity (PPB = 90.48%, H = 0.349 4, I = 0.509 6) and moderate genetic differentiation among populations (Gst = 0.260 9). Based on the unweighted pair-group method with arithmetic average (UPGMA), twelve populations gathered in three clusters. The cluster 1 included four populations. There are Nanjing, Zhenjiang, Xuancheng and Hangzhou. The seven populations which come from Hubei Province, Hunan Province, Jiangxi Province and Guizhou Province belonged to the cluster 2. The cluster 3 only contained Wenshan population. Moreover, Mantel test revealed significant positive correlation between genetic distances and geographic distances (r = 0.632 9; P < 0.000 1). According to the results, we proposed a series of conservation consideration for B. striata.
