New methods to detect autophagic flux
10.16438/j.0513-4870.2015-0877
- VernacularTitle:自噬流的检测方法
- Author:
Xiao-xi LÜ
1
;
Zhuo-wei HU
1
Author Information
1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study, Institute of Meteria Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
- Publication Type:SPECIAL REPORTS
- Keywords:
autophagic flux;
flow cytometry;
tandem fluorescent;
long lived protein;
microtubule-associated protein 1A/1B-light chain 3
- From:
Acta Pharmaceutica Sinica
2016;51(1):45-
- CountryChina
- Language:Chinese
-
Abstract:
Autophagy is a crucial biological process of eukaryotes, which is involved in cell growth, survival and energy metabolism, while the premise of the autophagy function is activated autophagic flux. It has been confirmed that impaired autophagic flux promotes pathogenesis of many chronic inflammatory diseases, especially cancer, neurodegenerative disease and tissue fibrosis, therefore the analysis of autophagic flux state is important for revealing autophagy function and the mechanism of autophagy related diseases. Given that autophagy is a dynamic process with multiple steps, it is very hard to observe the real state of autophagic flux. Summarized here is the novel concept and current approach to detect autophagic flux. This knowledge is crucial for the researching of the biological function of autophagy, and may provide some strategies for developing autophagy-related drug.
