Recent advances of quantitative detection methods for HBV cccDNA
10.3969/j.issn.1001-5256.2019.06.006
- VernacularTitle:HBV cccDNA定量检测方法的研究进展
- Author:
Peixue JIANG
1
;
Richeng MAO
;
Jiming ZHANG
Author Information
1. Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China
- Publication Type:Research Article
- Keywords:
hepatitis B virus;
cccDNA;
quantitative detection method
- From:
Journal of Clinical Hepatology
2019;35(6):1201-1204
- CountryChina
- Language:Chinese
-
Abstract:
Elimination of HBV cccDNA from hepatocytes infected with chronic HBV virus is considered to be the key to eradicating HBV. Monitoring HBV cccDNA before, during, and after viral treatment is essential for routine treatment of patients with chronic hepatitis B. With the introduction of new anti-HBV treatment technologies and new drugs targeting HBV cccDNA, Accurate and sensitive HBV cccDNA assays are urgently needed to evaluate efficacy. In recent years, HBV cccDNA detection methods have achieved gratifying results in both traditional PCR methods and digital PCR methods popular in recent years. In this paper, the advances in HBV cccDNA quantitative detection by qPCR, Magnetic bead capture hybridization, rolling circle amplification combined with in situ PCR, digital PCR and digital PCR assay in single cells were reviewed.
