Application of in situ hybridization in the detection of hepatitis B virus nucleic acids and cccDNA
10.3969/j.issn.1001-5256.2019.06.005
- VernacularTitle:原位杂交技术在检测HBV核酸和cccDNA中的应用
- Author:
Tong XU
1
;
Xiaonan ZHANG
;
Zhenghong YUAN
Author Information
1. Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China
- Publication Type:Research Article
- Keywords:
hepatitis B virus;
cccDNA;
in situ hybridization
- From:
Journal of Clinical Hepatology
2019;35(6):1197-1200
- CountryChina
- Language:Chinese
-
Abstract:
In situ hybridization (ISH) is a new technique which combines molecular biology, histochemistry, and cytology. It can quantify and locate specific nucleic acids at the cellular and chromosomal levels and is widely used in virological research. ISH is of great significance for the detection of hepatitis B virus (HBV) nucleic acids (RNA and replicative intermediate DNA) and covalently closed circular DNA. This article reviews the development of ISH and its application in HBV research.
