Clinical application of covalently closed circular DNA detection techniques
10.3969/j.issn.1001-5256.2019.10.002
- VernacularTitle:HBV cccDNA检测技术与临床应用
- Author:
Yu ZHANG
1
;
Jingmin ZHAO
Author Information
1. Pathological Diagnosis and Research Center, The Fifth Medical Center of Chinese PLA General Hospital, Beijing 100039, China
- Publication Type:Research Article
- Keywords:
hepatitis B virus;
DNA, circular;
Blotting, Southern;
polymerase chain reaction;
in situ hybridization
- From:
Journal of Clinical Hepatology
2019;35(10):2140-2144
- CountryChina
- Language:Chinese
-
Abstract:
Covalently closed circular DNA (cccDNA) is the original template for hepatitis B virus (HBV) replication and is the basis of persistent HBV infection. Only when HBV cccDNA is completely eliminated can HBV be eradicated. Therefore, the detection of HBV cccDNA plays an important role in evaluating the antiviral outcome of chronic hepatitis B, assessing HBV clearance, clarifying occult HBV infection, studying the pathogenesis of hepatitis B, and developing new drugs, and HBV cccDNA detection techniques with high sensitivity and specificity are the basis for clinical application. With reference to related reports in literature and our own research work, this article reviews the development and clinical application of HBV cccDNA detection methods in recent years.
