Expression of hypoxia-inducible factor 1α in the pressure-side periodontium during orthodontic tooth movement in rats
10.12016/j.issn.2096-1456.2018.04.005
- Author:
HE Zhengquan
1
;
YANG Kai
2
Author Information
1. Department of Orthodontics,Changsha Stomatological Hospital
2. Department of Orthodontics, Beijing Stomatological Hospital
- Publication Type:Journal Article
- Keywords:
Orthodontics;
Tooth movement;
Periodontal tissue remodeling;
Hypoxia;
Inducible factor 1α
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2018;26(4):227-230
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To assess hypoxia-inducible factor 1α (HIF-1α) expression levels in the periodontal tissues of the pressure side during orthodontic tooth movement in rats.
Methods:A total of 50 male 6-week-old Sprague Dawley (SD) rats were randomly divided into 10 groups of 5 rats each. The upper left first molar was the experimental tooth and was pulled mesially with an orthodontic force of 10-15 g for 0, 1, 3, 6, or 12 h, or 1, 3, 7, 14, or 21 d. Routine five-micrometer paraffin-embedded tissue sections were processed for HE staining and immunohistochemical staining for HIF-1α. The Image-Pro Plus system was used to quantitatively analyze the stained slices. The expression of HIF-1α in the periodontal tissue of the pressure side changed during the process of orthodontic tooth movement.
Results:The expression of HIF-1α increased immediately after loading for 1 h, reached a small peak at 3 h, and then decreased. After 12 h, the expression increased again, reached a peak after 1 d, and then gradually decreased to near the pre-loading level(P < 0.05).
Conclusion:There were differences in expression of HIF-1α in different groups in the periodontal tissues of the pressure side during orthodontic tooth movement in rats.
- Full text:大鼠正畸牙移动过程中压力侧牙周组织缺氧诱导因子-1α 的表达.pdf
