The identification of Sect. Cruciata (Gentiana) species using mtDNA nad1/b-c and nad5/d-e fragments
10.16438/j.0513-4870.2018-0716
- VernacularTitle:线粒体nad1/b-c及nad5/d-e在秦艽组植物中物种鉴定意义的评价
- Author:
Jia-ni LU
1
;
Zhi-li ZHAO
1
;
Liang-hong NI
1
;
Dorje GAAWE
2
;
Ma MI
2
Author Information
1. School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2. Tibetan Traditional Medical College, Lhasa 850000, China
- Publication Type:Research Article
- Keywords:
mtDNA;
italic>nad1 gene;
intron 2;
italic>Gentiana;
Sect. Cruciata;
DNA barcoding
- From:
Acta Pharmaceutica Sinica
2019;54(1):166-172
- CountryChina
- Language:Chinese
-
Abstract:
italic>Gentiana section Cruciata (Gentianaceae) is a medicinally important section of herbs, including Chinese traditional medicine Gentianae Macrophyllae Radix and Tibetan herb Jieji. Here, we assess the taxonomic significance using mtDNA nad1/b-c and nad5/d-e sequence data. A total of 144 nad1/b-c and nad5/d-e sequences from 11 species within Gentianaceae were obtained, including 138 sequences from 10 species within Gentiana section Cruciata and 6 sequences from Halenia elliptica (outgroup). The results showed that mtDNA nad1/b-c has species- level resolution within the section of Cruciata, i.e. the variable in the position 45 “C” could be used as a stable marker locus to distinguish G. robusta from other taxa; the variable in the position 352 and 353 “GA” could distinguish G. crassicaulis and G. tibetica from other taxa within the section. Intraspecies genotype variability was detected in nad1/b-c sequences of G. officinalis and G. siphonantha, respectively. These genotypes could be used as potential DNA barcode. In addition, intraspecies genotype variability was detected in nad5/d-e sequences of G. macrophylla, G. officinalis and G. siphonantha, respectively. Based on the stable marker locus, a species-specific PCR protocol was developed using the primer PF to identifying G. robusta in the section. This study could expand the understanding of the diversity of mtDNA nad1/b-c and nad5/d-e in the genus Gentiana, and provide the essence for the species identification within Gentiana section Cruciata.
