Expression of miR-21, miR-221, and miR-222 in exosomes of CAL27 tongue squamous cell carcinoma cells
10.12016/j.issn.2096-1456.2018.04.004
- Author:
HUANG Wenxi
1
;
OUYANG Ying
2
;
WEI Changbo
2
;
YU Dongsheng
2
Author Information
1. Department of Stomatology, Foshan Chancheng District Centre Hospital
2. Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology
- Publication Type:Journal Article
- Keywords:
Carcinoma;
Tongue;
Squamous cell;
Exosome;
miR-21;
miR-221;
miR-222;
qRT-PCR
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2018;26(4):222-226
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To assess the expression of miR-21, miR-221, and miR-222 in exosomes of CAL27 tongue squamous cell carcinoma cells.
Methods : CAL27 tongue squamous cell carcinoma cells and normal human oral keratinocytes (HOKs) were cultured, and then, the cultured supernatant was collected to separate the exosomes. Exosomes were detected by electron microscopy, and the expression levels of miR-21, miR-221, and miR-222 in the exosomes of tongue cancer cells were measured by qRT-PCR.
Results:Exosomes existed in the cultured supernatants of CAL27 cells and HOKs. Additionally, the expression levels of miR-21, miR-221, and miR-222 in the exosomes of CAL27 cells were significantly enhanced compared with those in the HOK exosomes (P < 0.05).
Conclusion :The expression levels of miR-21, miR-221, and miR-222 were markedly enhanced in the exosomes of CAL27 tongue squamous cell carcinoma cells.
- Full text:舌鳞状细胞癌细胞株CAL27 外泌体源性miR-21、miR-221 和miR-222 的表达.pdf
