Effect of PNPLA3 I148M mutation on expression of TGF-β1 in rat hepatic stellate cells
10.3969/j.issn.1001-5256.2016.04.035
- VernacularTitle:PNPLA3 I148M基因突变对大鼠肝星状细胞转化生长因子β1表达的影响
- Author:
Baiquan AN
1
;
Yongning XIN
;
Linlin LU
Author Information
1. Qingdao Medical College of Nanjing Medical University, Qingdao, Shandong 266021, China
- Publication Type:Research Article
- Keywords:
liver cirrhosis;
transforming growth factor beta1;
phospholipases;
point mutation
- From:
Journal of Clinical Hepatology
2016;32(4):769-771
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the mechanism of action of PNPLA3 I148M mutation in the development and progression of non-alcoholic fatty liver fibrosis. MethodsThe lentiviral vectors carrying the mutant or wild-type PNPLA3 I148M gene were constructed and transfected into rat hepatic stellate (HSC-T6) cells. Quantitative real-time PCR was applied to measure the mRNA expression of transforming growth factor β1 (TGF β1). The t-test was applied for statistical analysis. ResultsThe lentiviral vectors carrying the mutant or wild-type PNPLA3 I148M gene were successfully constructed and transfected into HSC-T6 cells, and a HSC-T6 cell line with stable expression of the mutant or wild-type PNPLA3 gene was established. Compared with the cell line carrying the wild-type gene, the cell line carrying the mutant gene showed significantly higher mRNA expression of TGF β1 (1.25±0.15 vs 0.48±0.07; t=11.826, P＜0001). ConclusionPNPLA3 I148M mutation can increase the expression of TGF β1 in HSC-T6 cells, which provides a new cell model and new research ideas for investigating the role of PNPLA3 I148M mutation in non-alcoholic fatty liver fibrosis.
