Effect of protein acetylation on hepatitis B virus replication
10.3969/j.issn.1001-5256.2016.08.013
- VernacularTitle:蛋白质乙酰化对HBV复制的影响
- Author:
Xiaofang JIA
1
;
Xiaonan ZHANG
;
Ao WU
Author Information
1. Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China
- Publication Type:Research Article
- Keywords:
hepatitis B virus;
acetylation;
trichostatin;
nicotinamide
- From:
Journal of Clinical Hepatology
2016;32(8):1497-1501
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of protein acetylation in host cells on the replication of hepatitis B virus (HBV) in hepatocytes, since HBV infection greatly threatens human health and the acetylation of encoding proteins in infected cells plays an important role in HBV replication and infection. MethodsThe deacetylase inhibitors trichostatin A (TSA) and nicotinamide (NAM) were used to stimulate HBV replication in HepG2.2.15 and HepAD38 cells, and the HBV replication markers were measured. The pan-acetylysin protein and Ac-H3 were examined by Western Blot. ResultsThe stimulation of cells with TSA and NAM increased the overall acetylation level of proteins in cells, and the acetylation level increased in a time- and dose-dependent manner. In the HepG2.2.15 and HepAD38 cells, stimulation with TSA and NAM reduced HBsAg level in the supernatant of cell culture and increased HBV DNA level in a time- and dose-dependent manner, while HBeAg in the supernatant of cell culture and DNA in cells did not change significantly. ConclusionAcetylation of host proteins may be involved in and affect HBV replication in cells, and further analysis and determination of host proteins whose acetylation affects HBV replication in cells help to learn more about the regulation of HBV replication and provide new thoughts for the development of specific antiviral strategies.
