Detection of Allergen Specific IgE by AdvanSure Allergy Screen Test.
10.3343/kjlm.2010.30.4.420
- Author:
Eun Jee OH
1
;
Sun Ah LEE
;
Jihyang LIM
;
Yeon Joon PARK
;
Kyungja HAN
;
Yonggoo KIM
Author Information
1. Department of Laboratory Medicine, School of Medicine, the Catholic University of Korea, Seoul, Korea. yonggoo@catholic.ac.kr
- Publication Type:Original Article ; Comparative Study ; English Abstract ; Evaluation Studies
- Keywords:
Allergens;
Specific IgE assay;
Allergy screen test;
ImmunoCAP assay
- MeSH:
Adolescent;
Adult;
Aged;
Allergens/diagnostic use/*immunology;
Child;
Child, Preschool;
Female;
Humans;
Hypersensitivity, Immediate/diagnosis;
Immunoglobulin E/*blood;
Immunologic Tests/methods;
Infant;
Male;
Middle Aged;
Reagent Kits, Diagnostic
- From:The Korean Journal of Laboratory Medicine
2010;30(4):420-431
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: In vitro serum allergen-specific IgE tests have been routinely used in the clinical diagnosis of allergic diseases. We evaluated the clinical usefulness of a newly introduced multiple antigen screen test, Advansure Allergy Screen (LG Life Science, Korea) (LG-Screen) for the detection of allergen specific IgE. METHODS: A total of 180 sera (80 for inhalant and 100 for food panels) were tested by LG-Screen and RIDA Allergy Screen (R-biopharm, Germany) (RIDA-Screen) assays. According to the 58-60 specific allergens or allergen groups, the positive rates and agreement rates were analyzed using the cut off levels of class 2. For the quantitation of total IgE and specific IgE, nephelometry and ImmunoCAP test were performed in the sera showing discrepant results between the two allergy screen assays. RESULTS: The agreement rate and kappa value (k) of total IgE between the two allergy screen assays was 73.9% and 0.333. LG-Screen showed higher agreement rate with nephelometry than RIDA-Screen. The positive rates to common outdoor inhalant and food allergens were significantly higher in RIDA-Screen. Overall agreement rate of specific IgE between the two allergy screen assays for 58 allergens was 86.7% (6,086/7,020) (k, 0.293). In samples showing discrepant results between the two allergy screen assays, concordance rate of allergy screen assay with ImmunoCAP assay was 70.9% (449/633) for LG-Screen (k, 0.585) and 29.1% (184/633) for RIDA-Screen (k, -0.303). CONCLUSIONS: LG-Screen showed a favorable agreement with RIDA-Screen and ImmunoCAP assays, and it could be used for the detection of allergen specific IgE in the clinical laboratory.
