Effect of TLR gene polymorphisms on primary immune response to hepatitis B vaccine in Han children of Guangxi
10.16462/j.cnki.zhjbkz.2019.04.006
- Author:
Hai LI
1
;
Ying-nan LV
;
Qing-li YANG
;
Yu JU
;
Qin-yan CHEN
;
Chao TAN
;
Bai-qing DONG
;
Qi-jun WU
Author Information
1. Department of Epidemiology, School of Public Health and Management, Guangxi University of Chinese Medicine, Nanning 530200, China
- Publication Type:Research Article
- Keywords:
Toll like receptor;
Single-nucleotide Polymorphism(SNPs);
Hepatitis B vaccine;
Immune response
- From:
Chinese Journal of Disease Control & Prevention
2019;23(4):397-401,411
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the association between Toll-like receptors(TLR) gene polymorphisms and the primary immune response level to Hepatitis B Vaccine in Han children in Guangxi. Methods A total of 513 Han children aged 8-9 months were collected from the department of pediatrics in the Maternal and Child Hospital of Guangxi Zhuang Autonomous Region and Nanning Maternal and Child Health Hospital from 2014 to 2016. Peripheral venous blood of each study object was collected to detect HBsAg, anti-HBs, HBeAg, anti-HBe, anti-HBc and HBV DNA. The polymorphisms of 10 sites of TLR gene were detected by SNPscanTM multiple SNP typing techniques. The association between allele, genotype of TLR gene and anti-HBs levels were analyzed by non-conditional logistic regression. Results The genetic polymorphism of TLR3 gene rs13126816 was related to immune response after primary Hepatitis B immunization in Han children in Guangxi (OR=1.79，95% CI: 1.11-2.89, P=0.018). The anti-HBs level of children with A/A genotype［238.04(519.75) mIU/L］and G/A genotype［347.96(619.68) mIU/L］were significantly lower than those with G/G genotype［489.08(854.76) mIU/L］, and the differences were statistically significant (all P<0.05). Anti-HBs level of children carrying allele A ［317.20(608.72) mIU/L］was significantly lower than those carrying allele G［457.01(852.66) mIU/L］, and the difference was statistically significant (Z=-3.055， P<0.05). The rest of the TLR genes were not related to the immune response of Hepatitis B Vaccine (all P>0.05). Conclusions The allele A of TLR3 gene rs13126816 may be the influencing factor for the low response of primary immune response to Hepatitis B Vaccine in Han children.
