Detection of human Sarcocystosis using dried blood on filter papers: An Immunofluorescent Antibody Test

PS Sharudin; R Rahimi; B Latif; MK Kutty; A Muslim; AS Abdul Rahim; M Abdul Aziz; AS Mahfudz; Yal Lim; CC Heo; S Md Noor; J Hiu; J Hussaini

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Detection of human Sarcocystosis using dried blood on filter papers: An Immunofluorescent Antibody Test

Author: Sharudin, P.S. ¹ ; Rahimi, R. ¹ ; Latif, B. ¹ ; Kutty, M.K ² ; Muslim, A. ³ ; Abdul Rahim, A.S. ⁴ ; Abdul Aziz, M ¹ ; Mahfudz, A.S. ¹ ; Lim, Y.A.L ³ ; Heo, C.C. ¹ ; Md Noor, S. ⁵ ; Hiu, J ⁴ ; Hussaini, J. ¹
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1. Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Jalan Hospital Sungai Buloh, 47000 Selangor, Malaysia
2. Department of Medicine, Lincoln University College, Kelana Jaya, 47301 Selangor, Malaysia
3. Department of Parasitology, Faculty of Medicine, University of Malaya, Jalan Universiti, 50603 Kuala Lumpur, Malaysia
4. Department of Forensic Medicine, Hospital Queen Elizabeth, Kota Kinabalu, 88200 Sabah, Malaysia
5. Department of Forensic Medicine, Hospital Sungai Buloh, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia
Publication Type:Journal Article
From:Tropical Biomedicine 2019;36(2):453-458
CountryMalaysia
Language:English
Abstract: Sarcocystosis, a parasitic infection caused by a protozoa belonging to the genus Sarcocystis, is found worldwide in both and animals. Sarcocystis spp., require two animal hosts to complete their life cycle. The infection has gathered more global attention after recent outbreaks, especially amongst wester travellers to Malaysia. Other than sporadic cases and the current outbreaks, little information is available regarding human Sarcocystis infection in Malaysia. The present study aims to determine the prevalence of sarcocystosis among humans using an immunofluorescent antibody (IFA) test applied to dried blood on filter papers. A total of 200 blood samples were collected on filter papers from autopsy cases at two Malaysian hospitals: Sungai Buloh Hospital (peninsular Malaysia) and Queen Elizabeth Hospital (Malaysian Borneo). Antigens were prepared from bradyzoites harvested from positive goats’ muscle samples. Of the 200 samples, 32 (16%) had Sarcocystis antibodies that showed positive fluorescence reactions on filter papers. There was no significant difference (t-test, p value > 0.05) in prevalence rates between samples collected from autopsies at peninsular Malaysia and Malaysian Borneo. The results demonstrated that the filter paper technique can be used as one of the alternative serological tests in the diagnostic of human sarcocystosis.
Full text:8.2019my1029.pdf