Human Umbilical Cord Blood Mononuclear Cell Transplantation in Rats with Intrinsic Sphincter Deficiency.
10.3346/jkms.2010.25.5.663
- Author:
Joa Jin LIM
1
;
Jin Beum JANG
;
Ji Young KIM
;
Sung Hwan MOON
;
Chung No LEE
;
Kyung Jin LEE
Author Information
1. Cha Stem Cell Institute, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Urinary Incontinence, Stress;
Intrinsic Sphincter Deficiency;
Leak Point Pressure;
Human Umbilical Cord Blood Mononuclear Cells;
Stem Cells
- MeSH:
Animals;
Cells, Cultured;
Cord Blood Stem Cell Transplantation/*methods;
Humans;
Leukocytes, Mononuclear/*transplantation;
Rats;
Rats, Sprague-Dawley;
Treatment Outcome;
Urinary Incontinence, Stress/diagnosis/*physiopathology/*surgery;
Urologic Surgical Procedures/*methods
- From:Journal of Korean Medical Science
2010;25(5):663-670
- CountryRepublic of Korea
- Language:English
-
Abstract:
To evaluate the effectiveness of the human umbilical cord blood (HUCB) transplantation for the treatment of intrinsic sphincter deficiency (ISD), we analyzed the short term effects of HUCB mononuclear cell transplantation in rats with induced-ISD. ISD was induced in rats by electro-cauterization of periurethral soft tissue with HUCB mononuclear cell injection after 1 week. The sphincter function measured by mean leak point pressure was significantly improved in the experimental group compared to the control group at 4 weeks. (91.75+/-18.99 mmHg vs. 65.02+/-22.09 mmHg, P=0.001). Histologically, the sphincter muscle was restored without damage while in the control group it appeared markedly disrupted with atrophic muscle layers and collagen deposit. We identified injected HUCB cells in the tissue sections by Di-I signal and Prussian blue staining. HUCB mononuclear cell injection significantly improved urethral sphincter function, suggesting its potential efficacy in the treatment of ISD.
