Separation and identification of glycoprotein in human serum of Fragile X syndrome
- Author:
Anh Thi Lan Luong
;
Hoan Thi Phan
;
Phuong Thi Minh Nguyen
;
Dung Tien Nguyen
;
Chi Van Phan
- Publication Type:Journal Article
- Keywords:
Fragile X syndrome;
Fragile X Mental Retardation;
proteomics
- MeSH:
Fragile X syndrome;
Fragile X Mental Retardation;
proteomics
- From:Journal of Medical Research
2008;59(6):22-28
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Background: Fragile X Syndrome (FXS) is the most common cause of inherited mental retardation. The absence of Fragile X Mental Retardation (FMRP) in Fragile X syndrome changes other proteins. Objective: To detect changes of glycoprotein in human serum of Fragile X syndrome. Subject and methods: Affinity chromatography with lectin concanavalin A (ConA) used to receive glycoprotein. The collected glycoprotein was then separated using 2-D electrophoresis. The protein spots were further excised, trypsin digested, and analyzed by nano LC couple with ESI-MS/MS and identified by MASCOT v1.8 software. Results and conclusion: 5 glycoproteins showed the different expression levels in the serum of Fragile X syndrome. Haptoglobin, Ig-J were increased and ceruloplasmin, transferring, Ig kappa were decreased. Using affinity chromatography with lectin concanavalin A (ConA), glycoprotein was received and divided on 2 ways electrokinetic chromatography. The mixture protein was identified with a reliability of 99.5% by 2 ways liquid chromatography combined with continuous spectrum mass.
