Experimental study on the effect of hedysarum polybotys saccharides and selenizated hedysarum polybotys saccharides on oral squamous cancer cells in vitro
10.12016/j.issn.2096-1456.2019.12.002
- Author:
ZENG Sujuan
1
;
PENG Bo
2
;
CHENG Weidong
3
;
WEI Dongfeng
4
;
HUANG Wenyan
2
;
LI Yunyang
2
;
ZHAO Wanghong
5
Author Information
1. 1. Department of Stomatology, Nanfang Hospital, Southern Medical University 2.Department of Pediatric Dentistry, Stomatological hospital affiliated to Guangzhou Medical University
2. Department of Pediatric Dentistry, Stomatological hospital affiliated to Guangzhou Medical University
3. School of Traditional Chinese Medicine, Southern Medical University
4. Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences
5. Department of Stomatology, Nanfang Hospital, Southern Medical University
- Publication Type:Journal Article
- Keywords:
hedysarum polybotys saccharides;
selenizated hedysarum polybotys saccharides;
oral squamous cell carcinoma SCC25;
experiment in vitro;
proliferation;
apoptosis;
Fas/Fasl gene
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2019;27(12):757-762
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of hedysarum polybotys saccharides (HPS) and selenizated hedysarum polybotys saccharides (SE-HPS) on the oral squamous cancer cell line SCC25.
Methods:Different concentrations (0, 10, 25, 50, 100, 200, 400 μg/ml) of HPS and SE-HPS were added to SCC25 cells in the logarithmic growth stage. Cell proliferation was detected by the CCK-8 method, apoptosis was detected by flow cytometry, and apoptosis-related indexes were observed by RT-qPCR and Western blotting.
Results :The concentrations of HPS and SE-HPS inhibited the proliferation of SCC25 cells. The inhibitory effect of 50 μg/mL HPS and SE-HPS on the proliferation of SCC25 cells was the strongest and was time-dependent. The inhibition effect significantly increased within 48 h, and the effect was achieved after 48 h. At the plateau stage, SE-HPS inhibited the proliferation of SCC25 cells more strongly than HPS (P < 0.05). The results of flow cytometry showed that 50 μg/mL HPS and SE-HPS acted on SCC25 cells for 48 h, and the apoptotic rates were 25.8% and 30.8% respectively. Compared with the control group (0 μg/mL HPS and SE-HPS), the difference was statistically significant (P < 0.05). RT-qPCR and Western blotting showed that 50 μg/mL HPS and SE-HPS acted on SCC25 cells for 48 h, and the mRNA and protein expression levels of the apoptosis gene Fas/FasL were upregulated. The difference was statistically significant (P < 0.05).
Conclusion: Both HPS and SE-HPS can inhibit the proliferation of SCC25 oral cancer cells, but SE-HPS is superior to HPS and can induce apoptosis through the Fas/Fasl pathway.
- Full text:红芪多糖和硒化红芪多糖对口腔癌细胞作用的体外实验研究.pdf
