Analysis on molecular epidemiological characteristics of 38 HIV-1 positive seroconcordant couples in Yunnan Province
10.16462/j.cnki.zhjbkz.2019.03.004
- Author:
Jia-fa LIU
1
;
Jian-jian LI
;
Xue-mei DENG
;
Nian ZHANG
;
Bi-hui YANG
;
Mi ZHANG
;
Xing-qi DONG
Author Information
1. Yunnan Provincal Infectious Diseases Hospital/AIDS Care Center Laboratory, kunming 650301, China
- Publication Type:Research Article
- Keywords:
HIV;
Antibody concordant positive couples;
Molecular epidemiology
- From:
Chinese Journal of Disease Control & Prevention
2019;23(3):263-267
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the molecular epidemiological characteristics of 38 HIV-1 positive seroconcordant couples in Yunnan Province. Methods The samples of HIV-1 antibody screened initially and positive confirmed in the Laboratory of Yunnan Provincial Infectious Diseases Specialist Hospital from 2014 to 2016 and laboratory data were collected. 41 samples from HIV-1 positive seroconcordant couples were selected and sequenced by RT-nested-PCR amplification. After the sequences were aligned and spliced, the qualified pol gene region sequence was used to construct phylogenetic tree and analyze subtypes, and the gene distance differences between each positive couple were calculated. Results A total of 76 pol gene regions from 38 HIV-1 positive seroconcordant couples were obtained. The major gene subtypes included CRF08_BC (45,59.2%), CRF07_BC (18,23.7%) and CRF01_AE (7,9.2%), there were also C (4, 5.3%) and B (2, 2.6%). Phylogenetic tree analysis and gene distance calculation clearly showed that HIV transmission between relationship partners occurred in 9 HIV-1 positive seroconcordant couples. Conclusions As the proportion of sexual transmission of HIV has risen year by year, the transmission between husband and wife should be the focus of our attention. Late diagnosis of HIV infection is the main factor that causes the transmission between husband and wife. For special populations, monitoring and testing should be strengthened as soon as possible, so as to reduce the occurrence of transmission between AIDS couples.
