Aberrant histone modification in peripheral blood CD4+ T lymphocytes in oral lichen planus
10.12016/j.issn.2096⁃1456.2018.06.005
- Author:
SHEN Jun
1
;
YIN Cao
2
;
WANG Xuan
2
;
YE Huiming
1
;
JIANG Xiao
2
Author Information
1. Department of VIP, Stomatological Hospital, Southern Medical University
2. Department of Periodontal and Oral Mucosal Disease, Stomatological Hospital, Southern Medical University
- Publication Type:Journal Article
- Keywords:
Oral lichen planus;
Histone deacetylases;
Histone acetylation;
CD4+ T lymphocytes
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2018;26(6):365-369
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the histone acetylation level and histone deacetylase (HDAC) activity of peripheral blood CD4+ T lymphocytes in patients with oral lichen planus (OLP).
Methods :Twenty-three OLP patients were selected from August 2016 to January 2017 from the Stomatological Hospital, Southern Medical University. The diagnosis was confirmed by pathology, and the lesions were divided into a nonerosive OLP group (11 cases) and an erosive OLP group (12 cases). Ten healthy sex- and age-matched volunteers served as controls. Immunomagnetic beads were used to separate CD4+ T lymphocytes, and histones and nucleoproteins were extracted. The global histone H3/H4 acetylation levels and HDAC activity of CD4+ T lymphocytes from all subjects were detected by ELISA. The differences between the OLP and control groups were statistically analyzed.
Results:Global histone H3 hypoacetylation was observed in the OLP group compared with the control group (u = -2.410, P = 0.012). However, there was no significant difference in the serum CD4+ T lymphocyte histone H4 acetylation level between the OLP and control group (u = -1.412, P = 0.158). HDAC activity was significantly higher in the OLP group than in the healthy control group (F = 5.749, P = 0.023), and much higher HDAC activity was observed in the erosive group than in the nonerosive (P = 0.014) and healthy control groups (P = 0.001). The degree of histone H3 acetylation correlated negatively with increased HDAC activity in the OLP group (rs = -0.771, P < 0.001). There was no correlation between the level of histone H3 acetylation and HDAC activity in the healthy control group (rs = 0.382,P = 0.276). The histone H4 acetylation level in the OLP group showed no correlation with HDAC activity (rs = 0.149, P = 0.498), and the histone H4 acetylation level in the control group also showed no correlation with HDAC activity (rs = 0.527, P = 0.117).
Conclusion :Abnormal histone acetylation of CD4+ T lymphocytes in the peripheral blood of patients with OLP was identified and could be related to HDAC activity, suggesting that the epigenetic modification of histone acetylation may play a role in the pathogenesis of OLP.
- Full text:口腔扁平苔藓患者外周血CD4 T淋巴细胞组蛋白乙酰化的意义.pdf
