cAMP-responsive-element-binding protein promotes the differentiation of human stem cells from the apical papilla via inhibition of the TGF-β1 pathway
10.12016/j.issn.2096⁃1456.2018.07.004
- Author:
GU Xuening
1
,
2
;
QUAN Jiamiao
1
,
2
;
GUO Yuqing
1
,
2
;
LI Song
1
,
2
Author Information
1. Stomatologic Hospital &
2. College, Anhui Medical University, Key Laboratory of Oral Diseases Research of Anhui Province
- Publication Type:Journal Article
- Keywords:
cAMP-responsive-element-binding protein;
Transforming growth factor;
Stem cells from the apical papilla;
Odontoblast;
Differentiation;
Mineralization;
Runt-related transcription factor 2
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2018;26(7):428-433
- CountryChina
- Language:Chinese
-
Abstract:
Objective :This study aimed to investigate the effect of cAMP-responsive-element-binding protein (CREB) overexpression on the differentiation of human stem cells from the apical papilla (hSCAPs), stimulated by transforming growth factor- beta (TGF-β1).
Methods:Cells were isolated from human immature third molars via enzymatic digestion. Four experimental groups were set up: ①a control group, receiving normal mineralization inducer (α-MEM, 10% FBS, 10 mmol/L β-sodium glycerophosphate, 50 μg/mL vitamin C, 10 nmol/L dexamethasone); ② a TGF-β1 group, receiving normal mineralization inducer and 5 μg/mL TGF-β1; ③ a TGF-β1+LV-empty group, receiving normal mineralization inducer and the transfected empty virus vector with 5 μg/mL TGF-β1; and ④ a TGF-β1+ov-CREB group, receiving normal mineralization inducer and the transfected CREB-overexpressing viral vector, with 5 μg/mL TGF-β1. The transfected cells were cultured in odontogenic medium in the presence or absence of TGF-β1 for 2 weeks. Alizarin red staining was used to detect mineralized nodules, and the mRNA expression of the mineralization genes runt-related transcription factor 2 (RUNX2), dentin sialophosphoprotein (DSPP) and alkaline phosphatase (ALP) was measured by qPCR.
Results :Compared with the control group (1.12 ± 0.11), TGF-β1 inhibited the deposition of calcium minerals (0.67 ± 0.12) (P < 0.05) via hSCAPs and inhibited the mRNA expression of RUNX2 (0.60 ± 0.03), DSPP (0.43 ± 0.12) and ALP (0.69 ± 0.05) (P < 0.05). In contrast, overexpression of CREB attenuated the effect of TGF-β1 on hSCAPs, resulting in the development of a high number of mineralized nodules (1.27 ± 0.10) (P < 0.01) and increased RNA levels of RUNX2 (1.33 ± 0.07), DSPP (1.32 ± 0.11) and ALP (1.26 ± 0.03) (P<0.05) compared with those in the TGF-β1 group.
Conclusion:Overexpressed CREB promotes odontogenic differentiation of hSCAPs by interfering with TGF-β1.
- Full text:环磷酸腺苷反应元件结合蛋白调控转化生长因子β1对人根尖牙乳头干细胞分化的作用.pdf
