Role of miR-27a in the osteogenic differentiation of beagle maxillary sinus membrane stem cells
10.12016/j.issn.2096⁃1456.2018.08.002
- Author:
ZHANG Jing
1
;
ZHU Shuangxi
2
;
RONG Qiong
3
,
4
;
PENG Wei
2
;
LI Xiang
2
;
CHEN Songling
2
Author Information
1. Department of Stomatology, Clifford Hospital, Guangzhou University of Chinese Medicine
2. Department of Stomatology, The First Affiliated Hospital, Sun Yat-sen University
3. Department of Stomatology, The First People&rsquo
4. s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology
- Publication Type:Journal Article
- Keywords:
MiR-27a;
Maxillary sinus membrane stem cells;
Osteogenic differentiation;
RUNX2;
OPN
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2018;26(8):484-490
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the expression level of miR-27a during the osteogenic differentiation of beagle maxillary sinus membrane stem cells (MSMSCs) and explore the role of miR-27a in the osteogenic differentiation of MSMSCs.
Methods:Beagle MSMSCs were cultured in vitro. The expression level of miR-27a was detected via RT-PCR after an osteogenic inductive culture was prepared. The mRNA expression levels of Runx2 and OPN were examined via RT-PCR, and the protein expression levels of Runx2 and OPN were examined via Western blot after the cells were transfected with pre-miR-27a or anti-miR-27a. Finally, osteoprogenitor cells transfected with pre-miR-27a were composited with Bio-Oss particles and subcutaneously implanted into nude mice to form ectopic bone formation models, and then the inhibition of bone formation from miR-27a was observed in vivo.
Results:The expression level of miR-27a in the beagle MSMSCs decreased after osteogenic inductive culturing. The relative miR-27a levels were significantly decreased at day 1 (t=3.795, P=0.023), day 3 (t=4.493, P=0.011), day 7 (t=11.591, P < 0.001), day 14 (t=12.542, P < 0.001), and day 21 (t=5.621, P=0.008) compared with day 0. In addition, the expression levels of Runx2 mRNA (t=4.923, P=0.007) and protein (t=4.425, P=0.008) were reduced after the cells were transfected with pre-miR-27a. The expression levels OPN mRNA (t=5.253, P=0.006) and protein (t=5.132, P=0.006) were also reduced. In contrast, the mRNA expression levels of Runx2 (t=3.925, P=0.013) and OPN (t=3.712, P=0.019) were increased after the cells were transfected with anti-miR-27a, and bone formation was observed after the subcutaneous implantation of beagle MSMSCs composited with Bio-Oss in nude mice. Nevertheless, ectopic bone formation was inhibited by pre-miR-27a-transfected beagle MSMSCs composited with Bio-Oss (t=7.219, P=0.0020).
Conclusion : MiR-27a negatively regulates the osteogenic differentiation of MSMSCs.
- Full text:miR-27a调控比格犬上颌窦黏膜干细胞成骨分化的研究.pdf
