A standardized extract of Asparagus officinalis stem prevents reduction in heat shock protein 70 expression in ultraviolet-B-irradiated normal human dermal fibroblasts: an in vitro study.
10.1186/s12199-018-0730-3
- Author:
Ken SHIRATO
1
;
Jun TAKANARI
2
;
Tomoko KODA
3
;
Takuya SAKURAI
4
;
Junetsu OGASAWARA
5
;
Hideki OHNO
6
;
Takako KIZAKI
4
Author Information
1. Department of Molecular Predictive Medicine and Sport Science, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo, 181-8611, Japan. shirato@ks.kyorin-u.ac.jp.
2. Amino Up Chemical Co. Ltd, 363-32 Shin-ei, Kiyota, Sapporo, Hokkaido, 004-0839, Japan.
3. Faculty of Nursing, Tokyo Healthcare University, 2-5-1 Higashigaoka, Meguro, Tokyo, 152-8558, Japan.
4. Department of Molecular Predictive Medicine and Sport Science, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo, 181-8611, Japan.
5. Department of Health Science, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido, 078-8510, Japan.
6. Social Medical Corporation, The Yamatokai Foundation, 1-13-12 Nangai, Higashiyamato, Tokyo, 207-0014, Japan.
- Publication Type:Journal Article
- Keywords:
Asparagus officinalis L.;
Dermal fibroblasts;
Heat shock protein 70;
Photoaging;
Skin health;
Ultraviolet
- MeSH:
Asparagus Plant;
Cells, Cultured;
Female;
Fibroblasts;
drug effects;
radiation effects;
HSP70 Heat-Shock Proteins;
biosynthesis;
Humans;
Middle Aged;
Plant Extracts;
pharmacology;
Polymerase Chain Reaction;
Skin;
drug effects;
radiation effects;
Skin Aging;
drug effects;
radiation effects;
Telomere;
metabolism;
Ultraviolet Rays;
adverse effects
- From:Environmental Health and Preventive Medicine
2018;23(1):40-40
- CountryJapan
- Language:English
-
Abstract:
BACKGROUND:Heat shock protein 70 (HSP70) exhibits protective effects against ultraviolet (UV)-induced premature skin aging. A standardized extract of Asparagus officinalis stem (EAS) is produced as a novel and unique functional food that induces HSP70 cellular expression. To elucidate the anti-photoaging potencies of EAS, we examined its effects on HSP70 expression levels in UV-B-irradiated normal human dermal fibroblasts (NHDFs).
METHODS:NHDFs were treated with 1 mg/mL of EAS or dextrin (vehicle control) prior to UV-B irradiation (20 mJ/cm). After culturing NHDFs for different time periods, HSP70 mRNA and protein levels were analyzed using real-time polymerase chain reaction and western blotting, respectively.
RESULTS:UV-B-irradiated NHDFs showed reduced HSP70 mRNA levels after 1-6 h of culture, which were recovered after 24 h of culture. Treatment with EAS alone for 24 h increased HSP70 mRNA levels in the NHDFs, but the increase was not reflected in its protein levels. On the other hand, pretreatment with EAS abolished the UV-B irradiation-induced reduction in HSP70 expression at both mRNA and protein levels. These results suggest that EAS is capable to preserve HSP70 quantity in UV-B-irradiated NHDFs.
CONCLUSIONS:EAS exhibits anti-photoaging potencies by preventing the reduction in HSP70 expression in UV-irradiated dermal fibroblasts.
