Correlation study between accumulation of triterpenoids and expression of relative genes in Alisma orientale.
10.19540/j.cnki.cjcmm.20181226.006
- Author:
Ya-Min ZHANG
1
;
Feng-Ling SUN
1
;
Xue-Hua LU
1
;
Li-Sha LI
1
;
Xiao-Mei XU
1
;
Wen-Jin LIN
1
;
Rong-Qing XU
1
Author Information
1. Fujian Academy of Medical Sciences,Fujian Provincial Key Laboratory of Medical Testing Fuzhou 350001,China.
- Publication Type:Journal Article
- Keywords:
Alisma orientale;
FPPS;
HMGR;
correlation;
triterpenoids
- MeSH:
Alisma;
chemistry;
genetics;
Chromatography, Liquid;
Geranyltranstransferase;
genetics;
Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent;
genetics;
Phytochemicals;
analysis;
Plant Extracts;
Plant Proteins;
genetics;
Plant Tubers;
chemistry;
Tandem Mass Spectrometry;
Triterpenes;
analysis
- From:
China Journal of Chinese Materia Medica
2019;44(5):942-947
- CountryChina
- Language:Chinese
-
Abstract:
To research the correlation between accumulation of triterpenoids and expression of key enzymes genes in triterpenoid biosynthesis of Alisma orientale,the study utilized UPLC-MS/MS method to detect eight triterpenoids content in the tuber of A. orientale from different growth stages,including alisol A,alisol A 24 acetate,alisol B,alisol B 23 acetate,alisol C 23 acetate,alisol F,alisol F 24 acetate and alisol G,and then the Real time quantitative PCR was used to analyze the expression of key enzymes genes HMGR and FPPS in triterpenoid biosynthesis. Correlation analysis showed that there was a significant positive relation between the total growth of these eight triterpenoids and the average relative expression of HMGR and FPPS(HMGR: r = 0. 998,P<0. 01; FPPS: r = 0. 957,P<0. 05),respectively. Therefore,the study preliminarily determined that HMGR and FPPS genes could regulate the biosynthesis of triterpenoids in A. orientale,which laid a foundation for further research on the biosynthesis and regulation mechanism of triterpenoids in A. orientale.
