Determination of six compounds in Berberidis Cortex and comparative study of its different species.
10.19540/j.cnki.cjcmm.20181226.014
- Author:
Qi LI
1
;
Huan DU
1
;
Huan-Song WEN
2
;
Tong XU
3
;
Yu-Jie WANG
3
;
Xian-Rong LAI
3
;
Gang FAN
3
Author Information
1. School of Pharmacy,Chengdu University of Traditional Chinese Medicine Chengdu 611137,China.
2. Chengdu Push Bio-Technology Co.,Ltd. Chengdu 610045,China.
3. School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine Chengdu 611137,China.
- Publication Type:Journal Article
- Keywords:
Berberidis Cortex;
Berberis diaphana;
Berberis kansuensis;
Berberis vernae;
bufotenidine;
ferulic acid 4-O-β-D-glucopyranoside
- MeSH:
Berberine;
analogs & derivatives;
analysis;
Berberis;
chemistry;
classification;
Chromatography, High Pressure Liquid;
Phytochemicals;
analysis;
Plant Extracts;
analysis
- From:
China Journal of Chinese Materia Medica
2019;44(5):968-974
- CountryChina
- Language:Chinese
-
Abstract:
In order to clarify the characteristic components of Berberidis Cortex,the preparative liquid chromatography and spectral analysis methods were used to separate and identify the unknown components in the water extract of Berberidis Cortex. Two compounds were isolated and identified as bufotenidine and ferulic acid 4-O-β-D-glucopyranoside. They were both isolated for the first time from Berberidis Cortex and Berberis. In addition,an HPLC method was successfully established for simultaneously determination of six compounds in Berberidis Cortex,and chemometric methods were used to study the chemical differences among three main species of Berberidis Cortex. The results suggested that jatrorrhizine and bufotenidine are the main difference compounds among the three species.Compared with B. kansuensis and B. diaphana,B. vernae contains significantly more jatrorrhizine(P<0. 01),and the content of bufotenidine in B. vernae was significantly higher than that in B. kansuensis(P<0. 05). Considering these results,further research is necessary to reveal the pharmacological activities of bufotenidine and the pharmacodynamic differences between the three species. The results could provide a reference for quality control,the basic research on effective substances,and development of Berberidis Cortex.
