Cloning and expression analysis of LncRNA-RgAGT2 responding to consecutive monoculture in Rehmannia glutinosa.
10.19540/j.cnki.cjcmm.20181204.003
- Author:
Xiao-Ran WANG
1
;
Yu-Hong WANG
1
;
Wei-Xi LI
1
;
Zhen LI
1
;
Fang-Ming LIU
2
;
Zhong-Yi ZHANG
3
;
Xin-Jian CHEN
1
Author Information
1. College of Life Sciences,Henan Agricultural University Zhengzhou 450002,China.
2. College of Forestry,Henan Agricultural University Zhengzhou 450002,China.
3. College of Crop Sciences,Fujian Agriculture and Forestry University Fuzhou 350002,China.
- Publication Type:Journal Article
- Keywords:
LncRNA;
RACE;
Rehmannia glutinosa;
consecutive monoculture obstacle;
gene clone;
gene expression
- MeSH:
Cloning, Molecular;
Gene Expression;
RNA, Long Noncoding;
Rehmannia;
Transcriptome
- From:
China Journal of Chinese Materia Medica
2019;44(4):703-711
- CountryChina
- Language:Chinese
-
Abstract:
The consecutive monoculture obstacle is a major problem in the field of Rehmannia glutinosa( R. glutinosa),has severely declined the yield and quality of R. glutinosa. Here,using hi TAIL-PCR and RACE techniques,we have cloned the full-length transcript( 1 573 bp) of Unigene 29334＿All screened by DGE as a consecutive monoculture obstacle response gene of R. glutinosa. Based on ORF Finder prediction,all ORFs detected in the full-length transcript were less than 300 nt,which suggested that the above transcript was confirmed to be a long non-coding RNA( LncRNA). With alignment in R. glutinosa transcriptome,this LncRNA was partially homologous to alanine glyoxylate transaminase 2 gene( Rg AGT2),which was named LncRNA-RgATG2. To further explore the function of LncRNA-RgAGT2,we have examined expression patterns of LncRNA-RgAGT2 and Rg AGT2 at five critical development stages( seedling,elongation,pre-expanding,mid-expanding,late-expanding) in the first and second year replanting of R. glutinosa,respectively. The results indicated that LncRNA-RgAGT2,as a potential regulator,is possible to play a vital role in Rg AGT2 expression regulation. Meanwhile,LncRNA-RgAGT2 has presented significant variation in all development stages of R. glutinosa,which could be used as a " diagnostic label" to assess consecutive monoculture obstacle. This study,for the first time,showed that LncRNA was responsible for the response and regulation of consecutive monoculture obstacle,which would be a powerful supplement to reveal the molecular mechanisms of consecutive monoculture obstacle of R. glutinosa.
