C_(19)-diterpenoid alkaloids from Aconitum austroyunnanense.
10.19540/j.cnki.cjcmm.20180808.001
- Author:
Jiang HU
1
;
Tao LYU
1
;
Jian CAI
1
;
Xiu GAO
1
;
Li-Fang ZHANG
1
;
Nian-Hua JING
1
;
Tian-Feng PENG
1
;
Jun-You SHI
1
;
Shan-Hu HAO
2
Author Information
1. Institute of Characteristic Medicinal Resource of Ethnic Minorities,Qujing Normal University Qujing 655011,China.
2. Nuclear Medicine Department,General Hospital of Shenyang Military Region Shenyang 110016,China.
- Publication Type:Journal Article
- Keywords:
Aconitum austroyunnanense;
Ranunculaceae;
chemical constitutent;
cytotoxicity;
diterpenoid alkaloids
- MeSH:
Aconitum;
Alkaloids;
Diterpenes;
Molecular Structure;
Plant Roots
- From:
China Journal of Chinese Materia Medica
2019;44(4):717-722
- CountryChina
- Language:Chinese
-
Abstract:
Eight C_(19)-diterpenoid alkaloids( 1-8) were isolated from the ethyl acetate soluble fraction of 95% ethanol extract of the ground roots of Aconitum austroyunnanense through various column chromatographies on silica gel,ODS,Sephadex LH-20 and MCI gel.Their structures were elucidated as 14α-benzoyloxy-13β,15α-dihydroxy-1α,6α,8β,16β,18-pentamethoxy-19-oxoaconitan( 1),N-deethylaconitine( 2),spicatine B( 3),leucanthumsine A( 4),acofamine B( 5),macrorhynine B( 6),aconitilearine( 7),and ambiguine( 8) based on their chemical and physicochemical properties and spectroscopic data. Compound 1 was a new compound and alkaloids 2-8 were isolated from this plant for the first time. Some isolated alkaloids were tested in vitro for cytotoxic potential by employing the MTT method. As a result,alkaloid 1 exhibited weak cytotoxic activity against three tested tumor cell lines( A-549,He La,and Hep G2) with IC_(50) values less than 20 μmol·L~(-1).
