Effects of ginseng neutral polysaccharide on gut microbiota in antibiotic-associated diarrhea mice.
10.19540/j.cnki.cjcmm.20181129.002
- Author:
Yu-Li QI
1
;
Shan-Shan LI
2
;
Di QU
2
;
Li-Xue CHEN
1
;
Rui-Ze GONG
2
;
Kun GAO
1
;
Yin-Shi SUN
1
Author Information
1. College of Chinese Medicinal Materials,Jilin Agricultural University Changchun 130118,China Institute of Special Animal and Plant Sciences,Chinese Academy of Agricultural Sciences Changchun 130112,China.
2. Institute of Special Animal and Plant Sciences,Chinese Academy of Agricultural Sciences Changchun 130112,China.
- Publication Type:Journal Article
- Keywords:
antibiotic associated diarrhea;
ginseng neutral polysaccharide;
gut microbiota
- MeSH:
Animals;
Anti-Bacterial Agents;
Diarrhea;
Gastrointestinal Microbiome;
Mice;
Panax;
Polysaccharides
- From:
China Journal of Chinese Materia Medica
2019;44(4):811-818
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of ginseng neutral polysaccharide on gut microbiota composition and diversity as well as the therapeutic effect for antibiotic associated diarrhea( AAD) in mice. The water-soluble ginseng neutral polysaccharide( WGPN) was purified from water-soluble ginseng polysaccharides( WGP) by DEAE-sepharose fast flow column,which was obtained from the roots of Panax ginseng. AAD mice were induced by gastric gavage with lincomycin hydrochloride,followed by administration of normal saline( natural recovery group,NR) or WGPN( WGPN group) for one week. Body weight changes,psychosis and diarrhea status were observed and assessed. 12 h after the last administration,histological observation of ileum and 16 S rRNA high throughput sequencing analysis of intestinal contents were conducted to identify the effects of WGPN on AAD mice. The results showed that WGPN could alleviate the symptoms of diarrhea in mice,decrease the inflammation and edema of ileum,and increase the length of intestinal villi. As compared to NR mice,WGPN could increase the relative abundance of Lactobacillus,and significantly decrease the relative abundance of Bacteroides,Streptococcus,Ochrobactrum and Pseudomonas at the genus level. In conclusion,WGPN could improve the gut microecology by recovering the ileum structure and improving the diversity and composition of the gut microbiota in AAD mice.
