Identification of stable reference gene by qRT-PCR in Senecio scandens.
10.19540/j.cnki.cjcmm.20181204.011
- Author:
Li-Ping WANG
1
;
Jin LIANG
1
;
Qin-Qin SHEN
1
;
Qiang WANG
1
Author Information
1. Institute of Ecological Agriculture, Sichuan Agricultural University Chengdu 611130, China.
- Publication Type:Journal Article
- Keywords:
NormFinder;
Senecio scandens;
geNorm;
real-time quantitative PCR;
reference gene
- MeSH:
Gene Expression Profiling;
Genes, Plant;
Medicine, Chinese Traditional;
Plants, Medicinal;
genetics;
Reference Standards;
Reverse Transcriptase Polymerase Chain Reaction;
Senecio;
genetics;
Transcriptome
- From:
China Journal of Chinese Materia Medica
2019;44(3):465-471
- CountryChina
- Language:Chinese
-
Abstract:
As a traditional Chinese medicine, Senecio scandens is rich in important compounds such as flavonoid and sesquiterpenoid. Based on the transcriptome data of S. scandens, 15 candidate reference genes were selected including ABCT, ACT1, ACT2, ACT3, ACBP, ARF, ATPS, EF-H, EF-1α, ETIF, GAPDH, GTPB, MPS, UCE and 60S. Firstly, 9 candidate genes with relatively stable expressions such as ACT1, ACBP, ARF, ATPS, EF-1α, GAPDH, MPS, UCE and 60S were screened from different tissues of S. scandens by RT-PCR. Then, qRT-PCR was used to quantitatively analyze gene expression of these nine candidates in S. scandens with or without stress treatments. Further analysis of these gene expression data by geNorm and NormFinder showed that ACT1 exhibited the stablest expression in all samples and could serve as a reference gene for future study of S. scandens, and provide an endogenous control for gene expression analysis.
