Process optimization for extraction and purification of polysaccharides from Cistanche deserticola.
10.19540/j.cnki.cjcmm.20181129.004
- Author:
Xing-Hui XIAO
1
;
Hai-Xia GAO
2
;
Hong-Hui LI
2
;
Xiao-Hui JI
2
;
Xiao-Nan CUI
2
;
Gui-Fang LI
2
;
Yu-Xia LI
2
Author Information
1. College of Biology Science, Shihezi University Shihezi 832000, China College of Biology Science, University of Science and Technology of China Hefei 230000, China.
2. College of Biology Science, Shihezi University Shihezi 832000, China.
- Publication Type:Journal Article
- Keywords:
Cistanche deserticola;
macroporous resin AB-8;
polysaccharides;
protein removal;
purification
- MeSH:
Cistanche;
chemistry;
Plant Extracts;
chemistry;
Polysaccharides;
isolation & purification;
Temperature;
Water
- From:
China Journal of Chinese Materia Medica
2019;44(3):475-481
- CountryChina
- Language:Chinese
-
Abstract:
In this study, taking Cistanche deserticola in Xinjiang as the experimental material, the optimal process for extracting polysaccharides from C. deserticola with water extraction was studied by using single factor and orthogonal experiment. Its effects on protein removal and polysaccharides retaining were investigated by using Sevag, enzymatic method or combination of these two methods, so as to determine the optimal method for protein removal from polysaccharides of C. deserticola; the decolorization and purification methods such as macroporous resin of AB-8 and activated Carbon were used to determine the optimal process. The results showed that the extraction rate of polysaccharides from C. deserticola was 18.40% during the optimal process of the water extraction as follows: extraction temperature 75 ℃, extraction time 165 min and solid-liquid ratio 1∶55. The protein removal rate can reach 31.40% and polysaccharide retention rate can reach 96.00% under the optimal protein removal process: temperature 50 ℃, time 2 h, and papain dosage 0.2%. The decolorization rate of activated Carbon and macroporous resin called AB-8 was 80.37% and 86.43%, and the recovery rate of polysaccharides was 77.05% and 91.93%, respectively, suggesting that macroporous resin was more suitable for decoloration. Macroporous resin named AB-8 increased the purity of the polysaccharide crude extract from 67.70% to 84.80% under the following conditions: concentration of the sample 4 g·L~(-1), concentration of the eluent 60% ethanol, and the flow rate 1 mL·min~(-1), showing significant purification effect.
