Developing spectrum-toxicity relationship with rough set theory for hepatotoxicity material basis of Polygonum multiflorum.
10.19540/j.cnki.cjcmm.20181114.001
- Author:
Yan LIN
1
;
Rong XIAO
1
;
Lin-Zi YIN
2
;
Ping WU
1
;
Yue-Xin LIU
1
;
Chun LI
3
;
Li-Mei LIN
1
;
Duan-Fang LIAO
1
Author Information
1. Key Laboratory for Quality Evaluation of Bulk Herbs, College of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China.
2. College of Physics and Electronic, Central South University Changsha 410012, China.
3. Key Laboratory for Quality Evaluation of Bulk Herbs, College of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
2,3,5,4′-tetrahydroxy stilbene-2-O-β-D-glucoside;
Polygonum multiflorum;
hepatotoxicity;
rough set theory;
spectrum-toxicity relationship
- MeSH:
Chemical and Drug Induced Liver Injury;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
adverse effects;
Fallopia multiflora;
chemistry;
Humans;
Medicine, Chinese Traditional;
Phytochemicals;
adverse effects
- From:
China Journal of Chinese Materia Medica
2019;44(3):509-517
- CountryChina
- Language:Chinese
-
Abstract:
Idiosyncratic hepatotoxicity of Polygonum multiflorum has attracted a great attention in the world. The most toxic part of idiosyncratic hepatotoxicity was screened by MTT assay and flow cytometry, which was the 50% ethanol elute by macroporous adsorptive resins from alcohol-extraction of P. multiflorum. The fingerprints were collected by HPLC from 50% ethanol elute of crude and processed P. multiflorum from different habitats, then 14 common peaks were determined. Spectrum-toxicity relationship was analyzed by rough set theory(RST). Two main chemical components were predicted for idiosyncratic hepatotoxicity, in which TSG was the greater contributor. Idiosyncratic hepatotoxicity of TSG was tested in vitro, and the results indicated that TSG was the most important constituent contributed to idiosyncratic hepatotoxicity of P. multiflorum. The study showed the discovery of the main chemical components for idiosyncratic hepatotoxicity, and RST was effective for analyzing the spectrum-toxicity relationship, which could be a new method used in the effective/toxic constituents field of traditional Chinese medicine.
