Dynamic chromatopharmacokinetics model for components in Chinese medicine and validation in Buyang Huanwu Decoction.
10.19540/j.cnki.cjcmm.20180925.005
- Author:
Mei-Feng XIAO
1
;
Xiao-Peng DUAN
2
;
Kai-Wen DENG
3
;
Yan-Tao YANG
4
;
Wen-Long LIU
4
;
Fu-Yuan HE
4
Author Information
1. College of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China Hunan Provincial Key Laboratory of Drugability and Preparation Modification of Traditional Chinese Medicine Changsha 410208, China.
2. College of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China Supramolecular Mechanism and Mathematic-Physics Chracterization for Chinese Materia Medica, Hunan University of Chinese Medicine Changsha 410208, China.
3. The First Affinity Hospital, Hunan University of Chinese Medicine Changsha 410007, China.
4. College of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China Hunan Provincial Key Laboratory of Drugability and Preparation Modification of Traditional Chinese Medicine Changsha 410208, China Supramolecular Mechanism and Mathematic-Physics Chracterization for Chinese Materia Medica, Hunan University of Chinese Medicine Changsha 410208, China.
- Publication Type:Journal Article
- Keywords:
Buyang Huanwu Decoction;
chromatographic fingerprint;
chromatopharmacokinetic;
dynamic Chinese medicine;
mathematics model;
supermolecule;
total quantum statistical moment
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
pharmacokinetics;
Rats
- From:
China Journal of Chinese Materia Medica
2019;44(3):574-581
- CountryChina
- Language:Chinese
-
Abstract:
The Chinese medicine is mostly derived from plants or animals, highly polymorphic, with dynamic components which are reflected by the characteristic peaks and fingerprint peaks in chromatographic fingerprints. The chromatopharmacokinetics method for determined components is not applicable due to dynamic changes of chromatopharmacokinetics. Based on the preliminary study, dynamic pharmacokinetics mathematical model for multiple components in Chinese medicine was set up and verified by Buyang Huanwu Decoction as the model drug, applying the principle of the total quantum statistical moment(TQSM), superimposing or subtracting the relevant statistical parameters in blood samples and blank samples. This provided a new method for the chromatopharmacokinetic study of Chinese medicine. HPLC was used to determine the TQSM parameters in blood and blank sample fingerprints of Buyang Huanwu Decoction at each point, and the overall TQSM parameters of drug-containing blood sample and blank samples were obtained with addition calculation of TQSM; while the initial TQSM of the pure drug can be obtained with subtraction calculation. The metabolic and absorption equilibrium constants were calculated iteratively to a steady state using the estimated metabolic equilibrium constants, then the metabolic chromatopharmacokinetic parameters in rats were obtained: VUC_T 1.262×10~8 mAu·s, MRT_T 37.48 h, VRT_T 9.016×10~2 h~2, CL_T 25.79 mL·h~(-1)·kg~(-1), Vs 1.586×10~2 mL·kg~(-1), t_(T,0.5) 6.15 h, respectively. This suggested that 95% of the compounds in whole recipe were metabolized and secreted from the body after 0-96.33 h. The experiment verified that the established mathematical model and the total quantum moment statistics parameters can represent the dose-time relationship of Buyang Huanwu Decoction, which can be used to study on in vivo metabolism dynamics for Chinese medicine.
