Isolation,screening and identification of endophytic fungi and detection of its antifungal effects against Alternaria panax.
10.19540/j.cnki.cjcmm.20181106.003
- Author:
Chun-Yuan ZHOU
1
;
Xiang-Min PIAO
1
;
Mei-Xia YAN
1
;
Ying-Ping WANG
1
Author Information
1. Institute of Special Wild Economic Animals and Plants,Chinese Academy of Agricultural Sciences Changchun 130112,China.
- Publication Type:Journal Article
- Keywords:
Alternaria panax;
Chaetomium globosum;
antifungal effects;
biocontrol;
endophytic fungi
- MeSH:
Alternaria;
pathogenicity;
Antibiosis;
Chaetomium;
classification;
isolation & purification;
Endophytes;
isolation & purification;
Fungicides, Industrial;
Panax;
microbiology;
Plant Diseases;
prevention & control
- From:
China Journal of Chinese Materia Medica
2019;44(2):274-277
- CountryChina
- Language:Chinese
-
Abstract:
To obtain biocontrol fungus for Alternaria panax,the antifungal effects of one strain of endophytic fungi isolated from leaves of healthy ginseng were screened and evaluated by using dual-culture method,and the taxonomic assignment of the screened strain was identified based on the morphological characters and ITS sequence analysis. The results of dual-culture showed that one of the endophytes marked as FS-01 had good antifungal effects and the inhibitory rates of FS-01 strain to A. panax was( 60. 21±0. 12) %.The hyphae junction of the both strains,A. panax dissolved,broke and winded,while the hyphae of FS-01 strain remained normal. The inhibitory rates of non-sterilized FS-01 strain fermentation liqud was( 13. 94±0. 21) %. Strain FS-01 identified as Chaetomium globosum.