Rapid identification of chemical components in compound Nanxing acesodyne plaster by UPLC-Q-TOF-MS/MS.
10.19540/j.cnki.cjcmm.20181107.004
- Author:
Guo-Shuai LI
1
;
Yang MA
2
;
Ting GENG
2
;
Xia GAO
2
;
Wen-Zhe HUANG
2
;
Liang CAO
2
;
Wei XIAO
2
;
Zhen-Zhong WANG
2
Author Information
1. School of Pharmacy,Nanjing University of Chinese Medicine Nanjing 210023,China Jiangsu Kanion Pharmaceutical Co.,Ltd. Lianyungang 222001,China State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process Lianyungang 222001,China.
2. Jiangsu Kanion Pharmaceutical Co.,Ltd. Lianyungang 222001,China State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process Lianyungang 222001,China.
- Publication Type:Journal Article
- Keywords:
UPLC-Q-TOF-MS/MS;
chemical composition spectrum;
compound Nanxing acesodyne plaster
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
chemistry;
Phytochemicals;
analysis;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2019;44(2):298-307
- CountryChina
- Language:Chinese
-
Abstract:
The study aims to qualitatively analyze the chemical composition of compound Nanxing acesodyne plaster by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry( UPLC-Q-TOF-MS/MS). The analysis was performed on Agilent Zorbax SB-C_(18)( 4. 6 mm×250 mm,5 μm) column. The mobile phase consisted of methanol and 0. 2% formic acid-water was used as gradient elution. The flow rate was 1 mL·min~(-1) and column temperature was 30 ℃. The Mass spectrometry was acquired in both positive and negative ion modes using ESI. The components were identified by the precise mass-to-charge ratio,secondary fragmentation and other information combined with reference substance and literature data. As a result,58 compounds were identified and predicted,including alkaloids,flavonoids,coumarins,organic acids and lactone compounds,of which 12 compounds were verified by the reference substances. The results provide reference for the quality control of compound Nanxing acesodyne plaster,and lay the foundation for elucidating the active components mechanism.
