Dihydrobenzofuran Neolignans Isolated from Euonymus alatus Leaves and Twigs Attenuated Inflammatory Responses in the Activated RAW264.7 Macrophage Cells.
10.20307/nps.2016.22.1.53
- Author:
Na Hyun KIM
1
;
Min Hye YANG
;
Jeong Doo HEO
;
Sang Hyun SUNG
;
Eun Ju JEONG
Author Information
1. Gyeongnam Department of Environment & Toxicology, Korea Institute of Toxicology, 17 Jegok-gil, Munsan-eup, Gyeongnam 660-844, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Euonymus alatus;
Neolignan;
Anti-inflammation;
RAW264.7
- MeSH:
Cytokines;
Dinoprostone;
Euonymus*;
Interleukin-6;
Lignans*;
Macrophages*;
Necrosis;
Nitric Oxide;
Nitric Oxide Synthase Type II
- From:Natural Product Sciences
2016;22(1):53-59
- CountryRepublic of Korea
- Language:English
-
Abstract:
Anti-inflammatory effects of dihydrobenzofuran neolignans isolated from Euonymus alatus leaves and twigs were evaluated in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Six neolignans, (+)- simulanol (1), (+)-dehydrodiconiferyl alcohol (2), (-)-simulanol (3), (-)-dehydrodiconiferyl alcohol (4), (+)-dihydrodehyrodiconiferyl alcohol (5), threo-buddlenol B (6) effectively inhibited the production of nitric oxide (NO) induced by LPS, and the activity of iNOS. (-)-dehydrodiconiferyl alcohol (4), which showed the most potent inhibitory activity, attenuated the activity of iNOS enzyme and also the expression of iNOS and COX-2 proteins. The subsequent production of pro-inflammatory cytokines, interleukin-1β, interleukin-6, tumor necrosis factor-α and prostaglandin E2 were also inhibited by the pretreatment of RAW264.7 cells with (-)-dehydrodiconiferyl alcohol (4). These neolignans are thought to contribute to anti-inflammatory effects of E. alatus, and expected to be potential candidates to prevent/treat inflammation-related diseases.
