Identification of Antioxidative Constituents from Polygonum aviculare using LC-MS Coupled with DPPH Assay.
10.20307/nps.2016.22.1.64
- Author:
Hyeji SHIN
1
;
Hayeon CHUNG
;
Byoungduck PARK
;
Ki Yong LEE
Author Information
1. College of Pharmacy, Korea University, Sejong 339-700, Korea. kylee11@korea.ac.kr
- Publication Type:Original Article
- Keywords:
Polygonum aviculare;
Flavonoids;
LC-MS;
DPPH assay
- MeSH:
Catechin;
Chromatography;
Flavonoids;
Methanol;
Phenol;
Polygonum*;
Silica Gel
- From:Natural Product Sciences
2016;22(1):64-69
- CountryRepublic of Korea
- Language:English
-
Abstract:
A method for simultaneously identifying antioxidative compounds was developed using time-based LC-MS coupled with DPPH assay regardless of the time consuming process. The methanolic extract of Polygonum aviculare (Polygonaceae) showed significant DPPH radical scavenging activity. Time-based DPPH assay for simultaneous identification of active compounds from the extracts of P. aviculare was used. Major peaks of ethyl acetate fraction of P. aviculare showed high DPPH radical scavenging activity. A simple phenolic compound (1) and six flavonoids (2-7) were isolated from the ethyl acetate fraction of P. aviculare by silica gel and sephadex LH-20 column chromatography. The structures of seven compounds were determined to be protocatechuic acid (1), catechin (2), myricitrin (3), epicatechin-3-O-gallate (4), avicularin (5), quercitrin (6), and juglanin (7) based on the analysis of the 1H-NMR, 13C-NMR and ESI-MS data. All compounds exhibited significant antioxidant activity on DPPH assay and active compounds were well correlated with predicted one.
