Mechanism of " acupuncture for the opening of ATP sensitive potassium channel against cerebral ischemia reperfusion injury in rats.
10.13703/j.0255-2930.2018.12.017
- Author:
Lin HAN
1
;
Yang GAO
1
;
Xuhui WANG
1
;
Yanan ZHANG
2
;
Xiangyu ZHANG
1
Author Information
1. Acupuncture Department, the First Teaching Hospital of Tianjin University of TCM, Tianjin 300193, China.
2. Acupuncture Research Institute, the First Teaching Hospital of Tianjin University of TCM.
- Publication Type:Journal Article
- Keywords:
ATP sensitive potassium channel;
apoptosis;
cerebral ischemia reperfusion;
“Xingnao Kaiqiao” acupuncture therapy
- MeSH:
Acupuncture Points;
Animals;
Brain Ischemia;
Electroacupuncture;
KATP Channels;
Rats;
Rats, Sprague-Dawley;
Reperfusion Injury
- From:
Chinese Acupuncture & Moxibustion
2018;38(12):1319-1324
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the mechanism of "" acupuncture for the opening of ATP sensitive potassium channel (K) against cerebral ischemia reperfusion injury in rats.
METHODS:Eighty-four rats were randomly divided into a sham-operation group, a model group, an electroacupuncture (EA) group, an EA+K blocker group, 21 rats in each group. 10 μL intracerebral injection with glipizide (1 μmol/5 μL) was used in the EA+K blocker group. The cerebral ischemia reperfusion model was established by Zea Longa's suture method in the model group, the EA group and the EA+K blocker group. Rats in the sham-operation group were received the same surgery but without nylon filament insertion. Acupuncture (20 min a time) was performed at "Neiguan" (PC 6), "Shuigou" (GV 26) and "Sanyinjiao" (SP 6) in the EA group and the EA+K blocker group at 10:00 and 16:00 for 3 days, firstly 90 min after model establishment. EA (2 Hz/15 Hz, 1 mA) was connected at the affected "Neiguan" (PC 6) and "Sanyinjiao" (SP 6). The same fixation was used in the sham-operation group and the model group, without EA. Neurological function was assessed by Zausinger's neurologic assessment scale. 2, 3, 5-triphenyltetrazolium chloride (TTC) staining was used to detect infarct volume. Neurocyte apoptosis in the hippocampus was detected by flow cytometry and the protein expressions of B lymphocytoma-2 gene (Bcl-2) and B cell lymphoma factor-associated X protein (Bax) were measured by Western-blot.
RESULTS:In comparison with the model group, the neurological score of the EA group increased (<0.01); the infarction volume and the hippocampal neuron's total apoptosis rate of the EA group decreased (both <0.05); the protein expression of Bcl-2 and Bcl-2/Bax of the EA group increased (<0.05, <0.01); and the protein expression of Bax of the EA group decreased (<0.01). Compared with the EA group, the neurological score of the EA+K blocker group decreased (<0.05); the total apoptosis rate of hippocampus neurons of the EA+K blocker group increased (<0.05); the expression of Bcl-2 protein of the EA+K blocker group reduced (<0.05); the expression of Bax protein of the EA+K blocker group increased (<0.05).
CONCLUSION:"" acupuncture has brain protective effect on rats with focal cerebral ischemia reperfusion injury. The mechanism may be related to regulating the opening of K channels and decreasing the apoptosis of neurons.
