Extremely low frequency electromagnetic radiation enhanced energy metabolism and induced oxidative stress in Caenorhabditis elegans.
- Author:
Yong-Yan SUN
1
;
Ya-Hong WANG
1
;
Zhi-Hui LI
1
;
Zhen-Hua SHI
2
;
Yan-Yan LIAO
1
;
Chao TANG
1
;
Peng CAI
1
Author Information
1. Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
2. University of Chinese Academy of Sciences, Beijing 100049, China.
- Publication Type:Journal Article
- MeSH:
Adenosine Triphosphate;
metabolism;
Animals;
Caenorhabditis elegans;
radiation effects;
Electromagnetic Radiation;
Energy Metabolism;
Mitochondrial Proton-Translocating ATPases;
metabolism;
Oxidative Stress;
Reactive Oxygen Species;
analysis
- From:
Acta Physiologica Sinica
2019;71(3):388-394
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to determine the effects of extremely low frequency electromagnetic field (ELF-EMF) on energy metabolism and oxidative stress in Caenorhabditis elegans (C. elegans). Worms in three adult stages (young adult stage, egg-laying stage and peak egg-laying stage) were investigated under 50 Hz, 3 mT ELF-EMF exposure. ATP levels, ATP synthase activity in vivo, reactive oxygen species (ROS) content, and changes of total antioxidant capacity (TAC) were detected, and worms' oxidative stress responses were also evaluated under ELF-EMF exposure. The results showed that ATP levels were significantly increased under this ELF-EMF exposure, and mitochondrial ATP synthase activity was upregulated simultaneously. In young adult stage, worms' ROS level was significantly elevated, together with upregulated TAC but with a decreased ROS-TAC score indicated by principal component analysis. ROS level and TAC of worms had no significant changes in egg-laying and peak egg-laying stages. Based on these results, we concluded that ELF-EMF can enhance worm energy metabolism and elicit oxidative stress, mainly manifesting as ATP and ROS level elevation together with ATP synthase upregulation and ROS-TAC score decrease in young adult C. elegans.
