Activation of the JAK/STAT signal pathway may be involved in DNA damage of A549 cells induced by X-ray.
- Author:
Li-Qiao PENG
1
;
Cheng-Hao LI
2
;
Bing MAO
3
Author Information
1. Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610000, China.
2. Provincial-level Key Lab for Molecular Medicine of Major Diseases and the Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Gansu University of Chinese Medicine, Lanzhou 730000, China.
3. Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610000, China. maobing@medmail.com.cn.
- Publication Type:Journal Article
- MeSH:
A549 Cells;
DNA Damage;
radiation effects;
Humans;
Janus Kinase 2;
metabolism;
Receptors, Interleukin-6;
metabolism;
STAT3 Transcription Factor;
metabolism;
Signal Transduction;
Tumor Suppressor p53-Binding Protein 1;
metabolism;
X-Rays
- From:
Acta Physiologica Sinica
2019;71(5):698-704
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the relationship between the effects of different doses of X-rays on DNA damage and JAK/STAT signaling pathway activation in A549 cells. The A549 cells were radiated with X-rays at doses of 2, 4, and 8 Gy. The proliferation of A549 cells was detected by CCK8 method. The content of interleukin 6 (IL-6) in culture medium at different time points after irradiation was detected by enzyme-linked immunoassay, and the expression levels of IL-6 receptor (IL-6R) and p53 binding protein 1 (53BP1) were detected by immunofluorescent staining. The expression levels of JAK2, p-JAK2, STAT3 and p-STAT3 were detected by Western blot. The results showed that, compared with the control group, X-ray irradiation reduced the cellular proliferation, up-regulated the expression of 53BP1, increased the IL-6 content in the medium supernatant, and up-regulated the protein expression levels of IL-6R, JAK2, p-JAK2, STAT3, and p-STAT3. The above effects of X-ray irradiation were dose-dependent. These results suggest that the mechanism by which X-rays cause DNA damage in A549 cells may involve activation of the JAK/STAT signaling pathway.
